酶免疫组化技术检测MAGE-A3抗原在肺癌组织中的表达  

Detection of the expression of MAGE-A3 in lung carcinoma tissues by enzyme immunohistochemical methods

作　　者：向波[1] 陆倩雯[1] 韩秀晶[1] 林云恩[2] 杨羚[1] 范婷婷[1] 廖伟娇[1] 

机构地区：[1]广州医学院第一附属医院检验科,510120 [2]广州医学院第一附属医院病理科,510120

出　　处：《国际检验医学杂志》2011年第10期1022-1023,1026,共3页International Journal of Laboratory Medicine

基　　金：广州医学院第一附属医院科研基金(y200910gyfy);广州医学院青年基金资助项目(2010A08)

摘　　要：目的检测黑色素瘤抗原A3(MAGE-A3)在原发性肺癌组织中的表达情况,并探讨其临床意义。方法应用间接酶免疫组化技术检测MAGE-A3抗原在48例肺癌组织中的表达情况,并分析其与肺癌分型和淋巴结转移的关系。结果 48例肺癌患者中有29例(60.4%)为MAGE-A3抗原表达阳性,19例(39.6%)为MAGE-A3抗原表达阴性;肺腺癌、肺鳞癌、肺腺鳞癌和小细胞肺癌的阳性率分别为80.0%、31.3%、75.0%和33.3%;肺腺癌组的阳性率显著高于肺鳞癌组(χ2=9.744,P<0.05),淋巴结转移组和非淋巴结转移组的阳性率差异无统计学意义(χ2=0.001,P>0.05)。结论 MAGE-A3抗原在肺癌组织中有较高的表达率,肺腺癌组MAGE-A3的表达阳性率显著高于肺鳞癌组,肺癌淋巴结转移组和非淋巴结转移组的阳性率差异无统计学意义。Objective To detect the expression of MAGE-A3 in primary lung carcinoma tissues and explore its clinical significance. Methods Indirect enzyme immunohistochemical method was performed to detect the expression of MAGE-A3 in 48 cases of primary lung cancer tissues. The correlation between MAGE A3 and the histological types of primary lung cancer and lymph node metastasis. Results Among the 48 cases of patients with lung cancer, 29 （60. 4%） cases were positive with MAGE A3 and 19 （39.6 %） were negative. The positive rates of MAGE A3 in lung adenocarcinoma （LA）, lung squamous cell carcinoma（LSqCa）, lung adenosquamous carcinoma（LASCa） and small cell lung cancer（SCLC） were 80.0 %, 31.3 %, 75.0 % and 33.3%. The positive rate of MAGE-A3 in LA group was higher than that of LSqCa group（χ2= 9. 744,P〈0. 05） ,but there was no statistical difference of it between lymph node metastasis group and non-lymph node metastasis group（χ2 =0. 001 ,P〉0.05）. Conclusion The expres sion of MAGE-A3 was more frequent in lung carcinomas,especially in LA,the positive rate of which was higher than LSqCa,while the expression of MAGE-A3 was not significantly associated with lymph node metastasis.

关 键 词：黑色素瘤 肺肿瘤 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]