红笛鲷α-珠蛋白和β-珠蛋白基因的克隆及序列分析  

Molecular Cloning and Sequence Analysis of α- and β-Globin Gene of Hunphead Snapper (Lutjanus sanguineus)

作　　者：张新中[1] 鲁义善[1] 吴灶和[1] 简纪常[1] 

机构地区：[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室暨广东省高等学校水产经济动物病害控制重点实验室,广东湛江524025

出　　处：《广东海洋大学学报》2011年第3期1-6,共6页Journal of Guangdong Ocean University

基　　金：国家自然科学基金项目(40906073);广东省科技厅国际合作项目(2009B050700040)

摘　　要：应用已知的标签序列通过RACE-PCR和RT-PCR方法成功克隆红笛鲷（Lutjanus sanguineus）α-珠蛋白和β-珠蛋白基因的全长cDNA序列。α-珠蛋白和β-珠蛋白基因的cDNA全长分别为560和563 bp,开放阅读框分别为429和444 bp,各编码143和148个氨基酸,理论相对分子质量分别为15690和16400,理论等电点为7.79和6.61。氨基酸序列BLAST结果表明,红笛鲷α-珠蛋白基因和β-珠蛋白基因编码的氨基酸序列与其他鱼类的相似性分别在59%-79%和55%-80%之间。用邻接法（Neighbor-Joining）构建的α-珠蛋白基因和β-珠蛋白基因的系统发育树表明,红笛鲷与真鲷（Pagrus major）、金头鲷（Sparus aurata）和鰤（Seriola quinqueradiata）等亲缘关系较近。Full-length cDNA sequence of α-globin and β-globin gene of humphead snapper（Lutjanus sanguineus） were cloned by RACE-PCR and RT-PCR method from the known ESTs.The length of α-globin and β-globin gene are 560 bp and 563 bp respectively.The open reading frame of α-globin and β-globin gene contain 429 bp and 444 bp respectively,and encode 143 and 148 amino acids respectively.Relative molecular weight of α-globin and β-globin are 15 690 and 16 400.The theoretical isoelectric point of them are 7.79 and 6.61,respectively.Results of amino acid sequence analysis show that the similarity of α-globin and β-globin gene of humphead snapper compared with other fish are in the range of 59 %-79 % and 55 %-80 % respectively.Phylogenetic tree of α-globin and β-globin gene are built by neighbor-joining method and the results also show that humphead snapper has the closest homology with Pagrus major,Sparus aurata and Seriola quinqueradiata.

关 键 词：红笛鲷 Α-珠蛋白 Β-珠蛋白 基因克隆 基因序列 

分 类 号：Q959.483.3[生物学—动物学] Q75