基于近缘EST-SSR引物的企鹅珍珠贝养殖和野生群体的遗传标记  

Genetic Markers Studies Based on EST-SSR Primer Relative to Pteria penguin of Farming and Wild Populations

作　　者：王永丽[1] 王梅芳[1] 王爱民[2] 王嫣[2] 余祥勇[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524025 [2]海南大学海洋学院,海南海口570228

出　　处：《广东海洋大学学报》2011年第3期7-11,共5页Journal of Guangdong Ocean University

基　　金：广东省科技计划项目(2008B020800014);广东省海洋渔业科技推广专项项目(A200899c04);公益性行业(农业)科研专项(200903028)

摘　　要：利用近源物种马氏珠母贝的EST-SSR引物对企鹅珍珠贝DNA进行扩增,获得7对可扩增出目标条带的近缘分子标记,分析野生群体（YS）和养殖群体（YZ）企鹅珍珠贝的遗传多样性。结果显示,7个位点共获得26个等位基因;野生和养殖群体的等位基因数分别为3~5和3~4,平均期望杂合度（He）分别为0.623 5、0.496 8;平均观测杂合度（Ho）分别为0.431 8、0.302 8;HNUPM047位点的多态信息含量较低,为0.446 7,其他位点均在0.5以上,说明该遗传标记可提供比较丰富的遗传信息。表明养殖群体遗传多样性水平低于野生群体。Amplifying the DNA of Pteria penguin（R？ding） from the Pinctada martensii’s EST-SSR primers,7 pairs of utilizable EST-SSR markers were found.The DNA of wild individuals from Guangxi Weizhou Island（YS） and cultured individuals from Guangdong Liusha Bay（YZ） were amplified under the optimal reaction condition.And genetic diversity of the two populations was analyzed.The results showed that 26 alleles were detected across the 7 loci.The number of alleles for the two populations ranged from 3 to 5（3.7 on average） and 3 to 4（3.4 on average）,respectively.The expected heterozygosity（He） was 0.623 5 and 0.496 8,and observed heterozygosity（Ho） was 0.431 8 and 0.302 8 respectively.Polymorphism Information Content（PIC） values of two populations at all loci were exceeded 0.50 except for locus HNUPM047 suggesting high polymorphism at microsatellite markers.Compared with the wild populations,genetic variance in the cultivated population was lower.

关 键 词：企鹅珍珠贝 EST-SSR 遗传多样性 

分 类 号：Q959.215.4[生物学—动物学] Q75