过表达缝隙连接蛋白Cx43与人脑胶质瘤细胞U251侵袭性的研究  

A study of connexin Cx43 overexpression on U251 glioma cell invasion

作　　者：闻海兵[1] 邹树峰[1] 李嘉杰[1] 洪涛[1] 

机构地区：[1]南昌大学医学院第一附属医院神经外科,南昌330006

出　　处：《重庆医学》2011年第19期1881-1884,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30760286)

摘　　要：目的通过上调脑胶质瘤细胞U251的Cx43表达水平,检测其对U251侵袭能力的影响,为抑制肿瘤的恶性浸润提供新的治疗途径。方法通过构建缝隙连接蛋白Cx43真核表达重组质粒,并转染U251细胞,过表达U251细胞缝隙连接蛋白Cx43,RT-PCR及Weston-blot检测Cx43 mRNA及蛋白表达水平变化。用Traswell细胞培养板结合MTT法测定U251细胞侵袭能力的改变。结果 (1)成功构建pCMV-Cx43 cDNA重组质粒。(2)成功转染U251细胞并筛选稳定转染过表达Cx43细胞株。(3)Traswell细胞培养板结合MTT法,检测到过表达Cx43后U251细胞侵袭能力较正常组下降。结论过表达缝隙连接蛋白Cx43能抑制人脑胶质瘤细胞U251侵袭能力。Objective Through in vitro overexpression of connexin Cx43 on U251 glioma cell invasion studies.To provide a new therapeutic approach the inhibition of invasion of malignant tumor.Methods Connexin Cx43 by constructing eukaryotic expression plasmid and transfected into U251 cells overexpressing connexin Cx43,RT-PCR and weston-blot detected the expression of Cx43 mRNA levels.Traswell cell culture plates with MTT method and the invasive ability of U251 cells to change.Results 1.successfullyconstructed the recombinant plasmid pCMV-Cx43 cDNA 2.successfully transfected U251 cells and selected stably transfected cell lines overexpressing Cx43.3.TRASWELL cell culture plates with MTT assay after the overexpression of Cx43 U251 cell infiltration capacity than the control group decreased.Conclusion Over expression of connexin Cx43 can inhibit invasion of U251 glioma cells.

关 键 词：质粒转染 缝隙连接蛋白CX43 人脑胶质瘤细胞 侵袭性 

分 类 号：R739.41[医药卫生—肿瘤]