响应曲面法及其在微生物发酵工艺优化中的应用  

Response Surface Method and Its Application in the Microbial Fermentation Process Optimization

作　　者：袁辉林[1] 康丽华[1] 马海滨[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《安徽农业科学》2011年第16期9498-9500,9502,共4页Journal of Anhui Agricultural Sciences

基　　金：国家级星火计划项目(2005EA169010);广东省林业科技创新专项(2009KJCX004-03)

摘　　要：[目的]介绍响应曲面法及其在微生物发酵工艺优化中的应用。[方法]应用响应曲面法对微生物发酵的培养基配方、培养条件和补料工艺进行优化。[结果]响应曲面法克服了传统方法的局限性,对影响试验指标的各因子水平及其交互作用进行优化和评价,快速有效地确定微生物发酵工艺中多因子系统的最佳条件。[结论]为正确运用响应曲面法开展微生物发酵工艺优化研究提供了科学依据和理论指导。[Objective] The research aimed to introduce the response surface method（RSM） and its application in the microbial fermentation process optimization.[Method] By using RSM,the medium components,culture conditions and feeding technique of microbial fermentation were optimized.[Result] RSM overcame the limitations of traditional methods,optimized and evaluated every factor level which affected the test index and their interactions,quickly and effectively determined the optimum condition of multi-factor in the microbial fermentation process.[Conclusion] The research provided the scientific basis and theory guidance for carrying out the microbial fermentation process optimization research by correctly using RSM.

关 键 词：响应曲面法 发酵工艺 优化 

分 类 号：S188.4[农业科学—农业基础科学]