费约果外植体消毒处理方法  

Research on the Disinfection Method of Feijoa Explants

作　　者：刘洪章[1] 刘艳军[2] 杨恩芹[2] 杨静慧[2] 苗博瑛[1] 

机构地区：[1]天津市林业果树研究所,天津300112 [2]天津农学院园艺系,天津300384

出　　处：《安徽农业科学》2011年第16期9546-9547,共2页Journal of Anhui Agricultural Sciences

基　　金：天津市农业科技成果转化与推广项目(0803120)

摘　　要：[目的]获得无菌、生长正常的费约果外植体,从而进行组培快繁。[方法]通过选用费约果不同组织部位作为外植体,采用不同消毒方法及不同预培养的培养基来进行费约果的外植体制备试验,从中筛选出适合的条件。[结果]采用费约果新梢为外植体,用0.1%HgCl2消毒4 min后,在含有羧苄青霉素、硫酸链霉素附加DTT、Vc的1/2MS培养基上预培养21 d获得的外植体消毒效果最好。[结论]获得了生长健壮的费约果外植体,为其快速繁殖提供了技术依据。[Objective] The sterile and normal growth explants from feijoa was obtained for its rapid multiplication.[Method] The experiment in the preparation of the feijoa explants from different tissues treated with different disinfection methods and different pre-culture medium was carried out so that the appropriate condition was selected.[Result] It was the best method that the new branch of feijoa being taken as explants was pre-cultured in 1/2MS medium containing carbenicillin,streptomycin sulfate,DTT and Vc for 21 days after it was disinfected with 0.1% mercuric chloride for 4 minutes.[Conclusion] The technical basis of the rapid propagation of feijoa was provided through the obtaining of health explants.

关 键 词：费约果 外植体 消毒 组织培养 

分 类 号：S66[农业科学—果树学]