可溶性淀粉合成酶SSⅢ基因对马铃薯的遗传转化  

Genetic Transformation of Potato with Soluble Starch Synthase SSⅢ Gene

作　　者：杜宏辉[1,2] 文义凯[2] 张宁[2] 司怀军[1,2] 王蒂[1] 

机构地区：[1]甘肃省干旱生境作物学省部共建国家重点实验室培育基地,甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [2]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《基因组学与应用生物学》2011年第3期303-307,共5页Genomics and Applied Biology

基　　金：863计划项目(2006AA100107);国家自然科学基金项目(30871573)共同资助

摘　　要：马铃薯是淀粉生产中重要的农作物之一,而可溶性淀粉合成酶SSⅢ是可溶性淀粉合成酶的主要活性成分,通过基因工程的手段来研究SSⅢ基因在淀粉合成中的功能可以用于改良马铃薯淀粉的品质。本研究采用根癌农杆菌介导法将强组成型表达启动子CaMV35S驱动的可溶性淀粉合成酶SSⅢ基因的RNA干扰表达载体导入马铃薯栽培品种克新1号和克新4号中,获得了65株卡那霉素抗性植株。对抗性植株PCR检测结果表明,SSⅢ基因的干扰片段已整合到马铃薯基因组中,RT-PCR检测表明SSⅢ基因在转录水平上受到了明显抑制。该研究为马铃薯淀粉品质的改良奠定了基础。Potato is one of the most important crops in the production of starch.Soluble starch synthase SSⅢ is the main activity composition of soluble starch synthase.Therefore,it is possible to alter starch quality and quantity and investigate function of SSⅢ gene in potato starch synthesis via genetic engineering methods.Soluble starch synthase SSⅢ gene of RNA interference expression vectors driven by the constitutive expression promoter CaMV 35S were introduced into two potato cultivars Kexin1 and Kexin4 by Agrobacterium-mediated transformation method.Then 65 kanamycin-resistant plants were obtained from the resistant kanamycin.PCR detection showed that the interference fragment of SSⅢ gene was integrated into potato genome.RT-PCR analysis showed that the expression of SSⅢ gene was repressed apparently on the transcription level.These results should be pave the way for improvement of potato starch quality.

关 键 词：马铃薯 SSⅢ基因 遗传转化 PCR 

分 类 号：S532[农业科学—作物学] Q943.2[生物学—植物学]