实验性胃溃疡大鼠尿液的代谢组学研究  

Metabonomic Investigation of Urine from Experimental Gastric Ulcer Rats

作　　者：彭树灵[1] 刘晓伟[1] 张真瑞[1] 杨健[1] 

机构地区：[1]南方医科大学中医药学院中医诊断教研室,广东广州510515

出　　处：《中国实验诊断学》2011年第6期989-991,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(NO:30271567);广东省中医药管理局基金(NO:1040089)

摘　　要：目的探讨大鼠实验性胃溃疡发生与自愈过程中尿液代谢表型的变化,揭示其生物学特征。方法 40只Wistar雄性大鼠完全随机分为5组:对照组与模型1天、3天、7天1、0天组,每组8只;采用乙酸性胃溃疡模型,运用气相色谱-质谱(GC-MS)技术获得各组大鼠尿液的代谢物谱,应用主成分分析(PCA)及偏最小二乘法判别分析(PLS-DA)进行模式识别,寻找表征不同代谢状态的生物标记物,根据这些物质水平的变化阐明相关机制。结果各组尿液代谢物谱明显不同;PCA得分图显示各组区分良好,不同时间模型组沿一定的轨迹向对照组靠拢;PLS-DA获得了15种生物标记物,包括一些有机酸、脂肪酸与氨基酸;不同组的标记物水平呈动态变化。结论大鼠胃溃疡发生与自愈过程中出现了能量与物质代谢异常,以及神经调节紊乱。Objective To investigate the changes of the urinary metabolic phenotype during experimental gastric ulcer developing and spontaneously healing in rats,and try to reveal the biological features of them.Methods Forty male Wistar rats were randomly divided into 5 groups：control group and different time model groups（1 day,3 days,7 days and 10 days groups）,with 8 rats in each group.Acetic acid gastric ulcer was adopted.Metabolic spectra of urine in each group were analyzed with GC-MS technique,and then pattern recognition of principal component analysis（PCA） and partial least squares discriminant analysis（PLS-DA） was performed to discover biomarkers.Relative mechanisms were explained according to the changes of biomarkers levels.Results The urinary metabolites in each group were significantly different.All groups could be distinguished in PCA score plot and different time model groups were approaching control group in a certain way.Fifteen biomarkers were acquired by PLS-DA,including some organic acids,fatty acids and amino acids,and their levels changed dynamically in different groups.Conclusion The energy and material metabolisms were disordered,so was the neural regulation,during gastric ulcer developing and spontaneously healing in rats.

关 键 词：胃溃疡 大鼠 代谢组学 气相色谱-质谱(GC-MS) 主成分分析(PCA) 偏最小二乘法判别分析(PLS-DA) 

分 类 号：R573.1[医药卫生—消化系统]