槲寄生碱体外通过激活Caspase-3诱导涎腺腺样囊性癌细胞凋亡  

Inhibitory effects of mistletoe alkali on the ACC cells by active Caspase-3

作　　者：李亚娟[1] 邱心如[2,3] 周洪澜[4] 王医术[2] 

机构地区：[1]四平市中心人民医院血液科,吉林四平136000 [2]吉林大学病理生物学教育部重点实验室 [3]吉林大学药学院 [4]吉林大学第一医院

出　　处：《中国实验诊断学》2011年第6期998-999,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划(201015198);大学生创新计划(2010A73113)

摘　　要：目的证实槲寄生碱(mistletoe alkali,MA)通过激活Caspase-3诱导人涎腺腺样囊性癌细胞(ACC)凋亡。方法将ACC细胞分为三组,即对照组、2.24μg/ml槲寄生碱处理组和1.12μg/ml槲寄生碱处理组,采用荧光染色法和免疫组织化学染色方法,检测三组细胞中Caspase-3蛋白的表达情况。结果槲寄生碱组Caspase-3表达明显高于对照组(P<0.01),并且药浓度越高,Caspase-3表达越强。结论槲寄生碱可通过激活Caspase 3诱导ACC细胞凋亡,并呈剂量依赖性。Objective To study whether alkaloid from mistletoe induces apoptosis of cells of adenoid cystic carcinoma of salivary gland（ACC cell） via activation of caspase3.Methods the cells were divided into three groups（control group 2.24 μg/ml alkaloid group,1.12 μg/ml alkaloid group）,the expressive status of caspase3 in three groups were detected by fluorescence and immunohistochemistry staining,the expression of alkaloid groups is obviously higher than the control groups.Results The expression of caspase3 of alkaloid group was much higher than that of control groups（P0.01）,and the expression is stronger with the higher concentration of alkaloid.Conclusion alkaloid of mistletoe induces apoptosis of ACC cell via activation of caspase3 and the increased caspase3 activity is dose dependent.

关 键 词：槲寄生碱 CASPASE-3 ACC细胞 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]