抑癌基因ING1在乳腺癌中的表达及其临床意义  

Expression and the significance of ING1 mRNA in breast carcinoma

作　　者：李欣[1] 张丽杰[1] 任云会[2] 

机构地区：[1]哈尔滨医科大学附属第三医院内五科,哈尔滨150081 [2]大庆油田总医院龙南医院

出　　处：《实用肿瘤学杂志》2011年第3期207-211,共5页Practical Oncology Journal

基　　金：黑龙江省自然科学基金资助项目（D2005-34）

摘　　要：目的探讨ING1基因在乳腺癌组织中的表达及其对乳腺癌发生发展的作用。方法采用逆转录一聚合酶链反应（RT—PER）方法检测51例乳腺癌组织、30例癌旁正常组织、12例乳腺良性肿瘤组织中ING1基因mRNA的表达情况。分析乳腺癌组织中ING1mRNA的表达与临床病理特征的关系。结果乳腺癌组织中ING1mRNA的表达水平（0．5458±0．2310）低于癌旁正常组织（0．6625±0．1699）。ING1mRNA表达水平在癌组织与癌旁正常组织、良性肿瘤三组间比较的差异有统计学意义（P〈0．05）；ING1mRNA在乳腺癌组织中的表达水平与病理类型及淋巴结转移有密切关系（P〈0．05），与其他病理学特征无关（P〉0．05）。结论ING1mRNA表达水平低下可能与乳腺癌的发生发展及预后密切相关，有可能成为乳腺癌基因治疗的新靶点。Objective To investigate the mRNA expression of tumor suppressor gene ING1 in breast carcinoma and evaluate its clinical significance. Methods Reverse transcription polymerase chain reaction （ RT - PCR） was used to detect the mRNA expression of ING1 in 51 cases of breast carcinoma,30 adjacent normal tissues and 12 benign breast tumors. The relationship of ING1 mRNA expression to elinicopathological characteristics was statistically analyzed. Results （ 1 ） The expression of ING1 mRNA in breast carcinoma tissues （0. 5458 ± 0.2310） was lower than that in the adjacent normal tissues （ 0. 6625 ± 0.1699 ）. The expression of ING1 mRNA in groups of breast carcinoma tissues ,the adjacent normal tissues and the benign breast tumor tissues were significantly different（ P 〈0.05 ）. （2）ING1 mRNA expression in breast carcinoma tissues was related with pathological type and lymph node metastasis （P 〈 0.05 ）. ING1 mRNA expression in breast carcinoma tissues had no relevance with the other clinicopathological characteristics （ P 〉 0.05 ）. Conclusion The mRNA expression of ING1 is significant associated with tumor progression in breast carcinoma, and it may be considered as a valuable marker for the gene therapy of breast carcinoma

关 键 词：乳腺肿瘤 抑癌基因 ING1 逆转录-聚合酶链反应 

分 类 号：R737.9[医药卫生—肿瘤]