钙敏感受体对人单核巨噬细胞株THP-1细胞因子分泌功能的影响  

The effect of calcium - sensing receptor on the secretion of cytokine in the differentiated THP - 1 cells

作　　者：席玉慧[1] 李鸿殊[1] 王秀丽[1] 孟庆威[2] 林岩[1] 王丽娜[1] 张力[1] 余立侠[3] 

机构地区：[1]哈尔滨医科大学病理生理教研室,哈尔滨150081 [2]哈尔滨医科大学附属第三医院 [3]大庆油田总医院让北医院

出　　处：《实用肿瘤学杂志》2011年第3期215-218,共4页Practical Oncology Journal

基　　金：黑龙江省教育厅科学技术研究项目（11521078）

摘　　要：目的探讨钙敏感受体（Calcium sensing receptor，CaSR）对THP-1源性巨噬细胞分泌细胞因子能力的影响。方法首先将人单核细胞株THP-1用佛波脂（PMA，100nmol／L）诱导，使之转化为具有贴壁能力的巨噬细胞株THP-1（以下简称THP-1）。用CaSR特异激动剂氯化钆（GdCl3，1retool／L）处理细胞后，用ELISA法检测CaSR的激活对THP-1分泌细胞因子IL-1β、TNFα的影响，并利用Western Blotting方法检测NF—κB信号通路中p65、p50和IKBct蛋白的表达变化。结果（1）用GdCl，处理后，THP-1细胞上清液中IL-1β、TNF—α的含量明显升高，但该作用可被CaSR特异抑制剂NPS2390（10μmol／L）抑制；（2）在Gd3＋作用组，THP-1细胞中NF—μB—p65表达明显上调，同时伴有IKBet的减少；在NPS2390预处理组，Gd3＋所引起的NF—κB—p65和IκBα变化被抑制；各组NF—κB—p50变化不明显。结论CaSR的激活可能通过激活NF—κB信号通路促进THP-1源性巨噬细胞株分泌细胞因子IL-1β、TNF—α，提示CaSR可能参与涉及单核巨噬细胞激活的各种疾病。Objective To observe the function of calcium sensing receptor（CaSR） in differentiated THP - 1 （human acute monocytic leukemia cell line）cells. Methods In the present study,we induced and differentiated the monocyte - like THP - 1 ceils into maerophage - like THP - 1 cells with PMA. The secretion of cytokines was analyzed by ELISA. The expression of CaSR and NF - κB in the differentiated THP - 1 cells was identified by Western blotting. Results （ 1 ） We found that GdCI3 elevation stimulated both of IL - 113 and TNFet release in differentiated THP- 1 ceils. （2）GdC13 could increase the expression of NF- κB -p65 and decrease the expres- sion of NF - κB - IκBα. The effects of GdC13 were inhibited by NPS2390. Conclusion The activation of CaSR in macrophage -like THP - 1 ceils could commit to cytokine secretion via NF -κB signal transduction pathway. These results suggested that CaSR might be involved in a variety of pathological processes mediated by activated monocyte - macrophages,

关 键 词：钙敏感受体 THP-1细胞 细胞因子 NF—κB信号通路 

分 类 号：R73-34[医药卫生—肿瘤]