急性髓细胞白血病免疫分型特点及临床意义  

Features of immunophenotype of acute myeloid leukemia and its clinical significance

作　　者：杨艳丽[1] 耿英华[1] 李佳佳[1] 李骏[1] 张凤[1] 

机构地区：[1]蚌埠医学院第一附属医院血液病科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2011年第6期561-563,共3页Journal of Bengbu Medical College

基　　金：安徽省卫生厅医学科研基金资助项目(2010C082)

摘　　要：目的:探讨急性髓细胞白血病(AML)免疫分型特点及其临床意义。方法:采用流式细胞术(FCM)对145例AML患者进行白血病免疫分型检测。结果:145例AML患者中MPO、CD13、HLA-DR、CD33、CD34表达率分别为93.1%、90.3%、77.2%、71.7%、70.3%。CD14仅表达于急性粒单细胞白血病(M4)和急性单核细胞白血病(M5)。AML伴淋系抗原表达者以CD7(34.5%)最为多见,其中以预后较差的M5型表达率为最高,其次为CD19(10.3%),且在急性粒细胞白血病部分分化型(M2)中表达率最高。CD2仅表达于部分急性早幼粒细胞白血病(M3),共发现2例急性混合细胞白血病,同时有淋系和髓系抗原表达。结论:MPO、CD13、CD33为髓系最敏感的抗原,CD7、CD19、CD2淋系抗原在AML诊断及预后判断上有重要指导意义,FCM免疫分型对白血病的准确分型诊断,预后判断有重要的指导意义。Objective：To investigate the characteristics and clinical significance of immunophenotype in acute myeloid leukemia （AML）. Methods：Immunophenotype was carried out in 145 patients with AML using flow eytometry. Results：The expression of MPO, CD13 ,HLA-DR,CD33 and CD34 was 93.1% ,90.3% ,77.2% ,71.7% and 70.3% ,respectively. CD14 was expressed only in the M4 and M5 subtype. The lymphoid antigen expression was most commonly observed in CD7 （34.5%） ,especially in M5 subtype and CD19 （ 10.3 % ） ;CD2 was expressed only in part of M3 subtype. Two cases of acute hybrid leukemia were detected, expressing both myeloid and lymphoid associated antigen. Conclusions： MPO, CD13 and CD33 are sensitive myeloid associated antigens. The expression of CD7, CD19 and CD2 ,as well as the immunophenotype by flow cytometry ,is of great value in diagnosis and prognosis of AML.

关 键 词：白血病 髓 急性 流式细胞术 免疫分型 

分 类 号：R733.71[医药卫生—肿瘤]