不同生境中半红树植物抗氧化防御研究  

Antioxidant Defenses of Mangrove Associates in Divergent Hatitats

作　　者：李妮亚[1] 韩淑梅[2] 陈坚[1] 钟才荣[3] 赵江艳[1] 王春霞[1] 

机构地区：[1]海南师范大学,海南海口571158 [2]海南大学,海南海口570228 [3]海南东寨港国家级自然保护区管理局,海南海口571129

出　　处：《西北林学院学报》2011年第5期29-34,40,共7页Journal of Northwest Forestry University

基　　金：海南省自然科学基金资助项目(310104);海南师范大学植物学重点学科项目(HS-1-2011-07001)

摘　　要：选取5种半红树植物,研究了其在不同生境(潮间带和陆生)抗氧化防御系统的变化。结果表明,陆生的黄槿、许树O2.产生速率和莲叶桐H2O2含量均显著的高于其潮间带居群,从而使这3种半红树叶片中抗氧化酶类(SOD、APX、CAT、POD和GR)活性均高于其潮间带居群。而处于潮间带杨叶肖槿、阔苞菊(除SOD外)的SOD、APX、CAT活性显著高于其陆生居群,此时,H2O2含量要显著低于其陆生居群,而陆生的阔苞菊居群SOD、POD、GR活性高于其潮间带居群,因此,O2.产生的速率显著低于其潮间带居群。不同树种对不同生境有着不同抗氧化防御策略,总的趋势是,陆生的半红树抗氧化酶活性均要高于其潮间带居群,尤其是POD、GR活性在5种半红树中均显著高于其潮间带居群,暗示环境胁迫造成的选择压力导致半红树植物适应性的反应。高温、高光照、干旱是对陆生半红树的逆境胁迫,因此,上调抗氧化酶活性,以降低逆境对半红树的膜脂过氧化,减少电解质外渗,最终提高了半红树的抗逆性。Changes of the anti-oxidative enzyme systems and reactive oxygen species（ROS） of Hibiscus tiliaceus,Thespesia populnea,Hernandia nymphiifolia,Clerodendrum inerme and Pluchea indica at divergent habitats（intertidal and inland） were surveyed.The results showed that O ·2 production rate was typically higher at inland in leaves of H.tiliaceus and C.inerme than at intertidal populations,and an abrupt rise of H2O2 content occurred in the leaves of H.nymphiifolia at inland.Therefore,the activities of superoxide dismutase（SOD）,catalase（CAT）,ascorbate peroxidase（APX）,guaiacol peroxidase（POD） and glutathione reductase（GR） increased in the leaves of H.tiliaceus,C.inerme and H.nymphiifolia at inland,but with different patterns,T.populnea rapidly triggered antioxidant defense at intertidal,H2O2 level declined correspondingly.P.indica up-regulated antioxidant enzymes in ASC-GSH cycle,SOD,POD,GR,to scavenge active oxygen species,therefore,P.indica maintained higher capacity to detoxify O ·2 production rate at inland.The strategies of antioxidant defense ware different among mangrove associate species at divergent habitats.In conclusion,activity of the anti-oxidative enzyme systems of mangrove associate species at inland was typically higher than at intertidal populations（especially POD,GR）.indicating that the selection pressure form environmental stress resulted in the responses of habitat adaptability of mangrove associate species.Mangrove associate leaves up-regulated antioxidant enzymes under various environmental stresses（high temperature,radiation and drough） at inland,thus limited environmental stresses induced lipid peroxide and membrane permeability,which contributed integrity maintenance and tolerance of mangrove associates.

关 键 词：半红树 潮间带 陆生 抗氧化酶 活性氧 

分 类 号：S718.43[农业科学—林学]