羟基马桑毒素及其衍生物对粘虫体内GAD和GABA-T活性的影响  

Effects of Tutin and Its Derivatives on GAD and GABA-T Activities of Pseudelatia separata

作　　者：杜薇薇[1] 李孟楼[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北林学院学报》2011年第5期149-152,共4页Journal of Northwest Forestry University

基　　金：国家自然科学基金项目(30872032)

摘　　要：为了探明羟基马桑毒素及其衍生物(T1、T2、T3、T4)对粘虫神经系统的影响以及快速检测对比分析新合成的药物对试虫神经递质γ-氨基丁酸(GABA)相关酶谷氨酸脱羧酶(GAD)和γ-氨基丁酸转氨酶(GABA-T)活性的影响,通过胃毒和离体2种处理方式提取粗酶液并采用分光光度法对试虫酶活性进行测定。结果表明,经羟基马桑毒素及其衍生物处理后试虫的GAD酶活受到了抑制,而GABA-T活性被提高,这2种酶影响着试虫体内GABA的含量。离体测定的结果与胃毒处理后试虫体内酶活的变化相似,因此可以采用离体处理方法快速检测新药物对试虫的活性。To understand the effects of tutin and its derivatives（T1,T2,T3,T4） on the nervous system of Pseudelatia separata and quickly determine the activities of glutamic acid decarboxylase（GAD） andγ-aminobutyric acid transaminase（GABA-T） treated by new synthesized compounds,the crude enzymes were extracted from 3rd instar larvae and the spectrophotometry was used to assay the activities of enzymes.One method of enzyme activity determination was to feed the larvae with toxins,and the other is to add toxins into enzyme directly.The results showed that GAD activities were inhibited by tutin and its derivatives,but the GABA-T activities were stimulated,both of them were impacted by the contents of GABA.The enzyme activities determined by two methods were similar,indicating that the method of activities determination of enzymes adding toxins directly could be used on the activities quick determination of new compounds.

关 键 词：羟基马桑毒素 粘虫 谷氨酸脱羧酶(GAD) γ-氨基丁酸转氨酶(GABA-T) 活性测定 

分 类 号：S763.306.7[农业科学—森林保护学]