不同序贯的吉非替尼与化疗药物联合对人肝癌细胞Bel-7402增殖的抑制作用  

Sequence-Dependent Antiproliferative Effects of Gefitinib and Cytotoxic Drugs on Bel-7402 Cells

作　　者：魏超[1] 周乃珍[1] 郭慧[1] 朱联九[1] 张盛周[1] 

机构地区：[1]安徽师范大学生命科学学院生物环境与生态安全省级重点实验室,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2011年第3期265-268,共4页Journal of Anhui Normal University(Natural Science)

基　　金：安徽高校省级自然科学研究重点项目(KJ2007A119ZC);生物环境与生态安全省级重点实验室及安徽省重要生物资源保护与利用重点实验室建设基金(2007-10)

摘　　要：应用MTT法检测了靶向性药物吉非替尼(gefitinib)分别与阿霉素(ADM)、氟尿嘧啶(5-FU)、顺铂(DDP)和紫杉醇(PTX)等4种化疗药物按照不同给药序贯联合作用对肝癌细胞Bel-7402增殖的影响.结果表明,吉非替尼与4种化疗药物联用对Bel-7402细胞增殖的抑制作用效果均要好于单药治疗,其对细胞的IC50较单独用药时明显较低.联合用药效果受到给药序贯的影响,用靶向性药物前先用化疗药物的效果要好于先用靶向性药物后用化疗药物.研究结果可供肝癌临床治疗参考,有助于临床降低用药剂量,减轻药物毒副作用.The inhibitory effects of gefitinib combining with different concentrations of adriamycin,fluorouracil,cisplatin and paclitaxel in different sequences on the proliferation of Bel-7402 cells were evaluated by MTT assay.The results showed that the inhibitory effect of cytotoxic drug combining with gefitinib was better than that of cytotoxic drug treatment alone,the IC50 decreased significantly.The antiproliferative effects of drug combination were affected by different drug sequences,gefitinib used after cytotoxic drugs created better curative effects than that before cytotoxic drugs.These results provided helpful information for hepatocellular carcinoma clinical therapy,they could conduce to less doses and toxic effects in clinical application.

关 键 词：吉非替尼 化学治疗 肝癌 联合用药 

分 类 号：R735.7[医药卫生—肿瘤]