三十日龄Fmr1基因敲除小鼠的水迷宫实验观察  

Behavioural comparision on Fmr1 knockout mice at 30 days age in Morris water maze experiment

作　　者：孙卫文[1] 黄越玲[2] 张维雯[3] 刘国彬[1] 沈岩松[1] 李敏雄[1] 戴丽军[2] 陈盛强[1] 

机构地区：[1]广州医学院第二附属医院,广东广州510260 [2]广州医学院动物实验中心,广州510182 [3]广州医学院第三附属医院,广州510340

出　　处：《解剖学研究》2011年第1期13-16,共4页Anatomy Research

基　　金：广东省自然科学基金(815101700100005);广东省科技计划项目(2008B0303013712009B030801368);广州市中医药中西医结合科研立项项目(2008A52);广东省中医药管理局建设中医药强省科研基金(2008184);广州市医学科研立项项目(2008A52)

摘　　要：目的实验对30日龄的Fmr1基因敲除(KO)小鼠的经典Morris水迷宫实验进行观察。方法采用Morris水迷宫实验,测试1月龄KO小鼠与WT小鼠的学习记忆功能。水迷宫实验共训练4 d,记录每天的潜伏期与游泳轨迹,第5天去除平台,记录小鼠停留各象限的时间百分比。根据所获得的数据进行多因素方差分析处理。结果①空间航行实验第1天至第3天实验中KO鼠与WT鼠的潜伏期和穿越平台次数差异无统计学意义(P>0.05);在第4天实验中KO鼠的潜伏期和穿越平台次数比WT鼠差异有统计学意义(P<0.05)。②空间搜索实验4周龄WT鼠在目标象限停留时间比其它象限停留时间长;4周龄KO鼠在第二象限停留时间长。结论 30日龄KO小鼠存在认知功能障碍。Objective To compare the behaviour defferences at 30 days age in Morris water maze experiment.Methods Fmr1 knockout mice were identified using the PCR technique,and Morris water maze experiment were used in the study.The data was analyzed with Multifactor Variance Analysis.Results ①Space navigation experiment from the first day to the third day,KO mice have no obviously difference with the WT mice in the Latency and number of crossing platform（P 0.05）,but on the fourth day,there was a statistical significance（P 0.05）;②Space search experiment.The four-week WT mice will stay longer than the other mice at the target quadrants;the four-week KO mice stay at the second quadrant longer.Conclusion Fmr1 knockout animals exhibited low ability of learning and memorizing in the Morris water maze task at 30 days Age.

关 键 词：脆性X综合症 水迷宫实验 FMR1基因敲除 认知 行为学 

分 类 号：R749.94[医药卫生—神经病学与精神病学]