阳离子载基因MePEG-PLGA纳米粒的制备及优化处方的筛选  

Preparation and selection of optimization of cationic gene loaded MePEG-PLGA nanoparticles

作　　者：刘菲菲[1] 龙大宏[1] 

机构地区：[1]广州医学院人体解剖学教研室,广东广州510182

出　　处：《解剖学研究》2011年第2期95-100,共6页Anatomy Research

基　　金：广东省自然科学基金(9151063101000016);广州市科技局应用基础研究计划项目(2009J1-C361-2)

摘　　要：目的建立纳米粒沉淀法制备阳离子甲氧基封端的聚乙二醇-聚乳酸聚乙醇酸嵌段聚合物(MePEG-PLGA)纳米粒的方法。方法本研究利用单因素设计和正交实验选定最优实验方案,并对纳米粒的物理性质如表面形态,粒径分布、Zeta电位、DNA结合率,保护DNA能力进行考察。结果最优条件制备得到的纳米粒粒径大小为89.7 nm,表面电位为28.3 mV,透射电镜下的纳米粒颗粒分散,大小均匀,表面光滑,呈球形分布,DNA结合率为80%,并能很好地保护所载基因不受核酸酶降解。结论利用纳米粒沉淀法制备得到的阳离子纳米粒有望成为高效的基因载体。Objective To develop a nanoprecipitation method for preparation of cationic monomethoxy（polythylene glycol）-poly（D,L-lactic-co-glycolide） nanoparticles.Methods This studies aimed to select the best preparation solution of MePEG-PLGA nanoparticles by single factor investigation and orthogonal experiment,and to conduct ivestigations the physical properties such as sruface morphology,particle size distribution,Zeta potential,DNA binding efficiency,DNA protection ability.Results The average particle size by optimal conditions was 89.7 nm,Zeta potential was 28.3 mV.Nanoparticles under the electron microscope were particles dispersed,size uniform,smooth,spherical distribution.DNA binding efficiency was 80%,and DNA-MePEG-PLGA-NPs may very well protect gene from nuclease degradation.Conclusion The preparation of cationic nanoparticles by nanoprecipitation were expected to be efficient gene vectors.

关 键 词：聚乳酸-聚羟基乙酸共聚物 聚乙二醇 十六烷基三甲基溴化铵 纳米粒沉淀法 

分 类 号：R749.16[医药卫生—神经病学与精神病学]