三氧化二砷对人肝癌SMMC-7721细胞侵袭转移能力的影响  

Effect of Arsenic trioxide on invasion and metastasis of human hepatocarcinoma cells SMMC-7721 in vitro

作　　者：余桂芳[1] 陈益民[1] 严跃红[1] 曾文铤[2] 朱科伦[2] 

机构地区：[1]广州医学院港湾医院肝病区,广东广州510700 [2]广州医学院第一附属医院肝病研究室,广东广州510120

出　　处：《现代肿瘤医学》2011年第7期1282-1284,共3页Journal of Modern Oncology

基　　金：广州市中医药科研基金资助项目(编号:2010A34)

摘　　要：目的:探讨三氧化二砷(As2O3)对人肝癌SMMC-7721细胞黏附、侵袭、转移能力的影响以及其机制。方法:采用MTT法观察人肝癌SMMC-7721细胞经As2O3作用前后对基底膜黏附能力的影响;Transwell膜侵袭系统观察SMMC-7721细胞经As2O3作用前后游走与穿透基底膜能力的改变。免疫细胞化学方法检测人肝癌细胞经As2O3作用前后CD44V6表达的改变。结果:As2O3能显著抑制人肝癌SMMC-7721细胞与m arigel的黏附、抑制细胞游走与穿透基底膜的作用(P<0.05),能抑制人肝癌细胞CD44v6的表达(P<0.05)。结论:As2O3具有抑制人肝癌细胞的侵袭、转移能力,其抑制作用可能与CD44v6的表达下调有关。Objective：To study the effect of Arsenic trioxide（As2O3） on adhesion and invasion metastasis of SMMC-7721 human liver cancer cell in vitro.Methods：MTTwas used to study the effect of adhesion by As2O3 on SMMC-7721 cell.The Transwell Chamber membrane invasion culture system was used to study the invasion and metastasis.The protein of CD44v6 was determined by immunocytochemical（ICC） staining.Results：As2O3 could significantly inhibit SMMC-7721cell adhesion with matrigel,movement and migration through matrigel than the control（P0.05）.As2O3 could significanty inhibit the expression of CD44v6 than the control（P0.05）.Conclusion：As2O3 have the effect of antagonizing hepatocarcinoma cell adhesion and invasion metastasis,and inhibites human hepatocarcinoma cells adhering to the basal membrane by down regulation the protein expression of CD44v6.

关 键 词：肝癌 SMMC-7721细胞 三氧化二砷 黏附 侵袭 CD44拼接异构体6 

分 类 号：R73-361[医药卫生—肿瘤] R735.7[医药卫生—临床医学]