IL-1β对人肺成纤维细胞增殖及MMP-2表达的影响  

Influence of IL-1β on the expression of MMP-2 of human fetal lung fibroblasts cells

作　　者：杨娜[1,2] 朱运奎[2] 李继东[2] 刘杜娇[2] 刘卫[2] 李超然[1,2] 

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730050 [2]兰州军区兰州总医院呼吸内科,甘肃兰州730050

出　　处：《现代肿瘤医学》2011年第7期1285-1288,共4页Journal of Modern Oncology

基　　金：甘肃省自然科学研究基金资助项目(编号:SZS051-A25-088)

摘　　要：目的:建立人肺成纤维(HFL)细胞三维立体培养模型,探讨IL-1β对HFL细胞MMP-2表达及细胞增殖的影响。方法:采用HFL细胞建立三维立体培养模型,随即分成对照组与实验组,对照组用不含IL-1β的DMEM培养,实验组分别加入含不同浓度的IL-1β(10,20 ng/m l)的DMEM培养液,用倒置显微镜观察细胞的形态,培养24、72h后收集培养上清液,应用明胶酶谱法测各组MMP-2的表达情况;利用MMT比色实验观察IL-1β对HFL细胞增殖的影响。结果:观察三维胶原立体培养中细胞生长情况,HFL细胞均悬浮生长于胶原中,细胞在胶原内几乎不分裂,呈单细胞生长。各实验组的HFL细胞在不同浓度的IL-1β作用下,细胞表达的MMP-2有差异(P<0.05),且随着IL-1β浓度的增大,表达MMP-2的量增加。IL-1β可促进HFL细胞的增殖(P<0.05),且具有浓度及时间依赖性,随着IL-1β的浓度升高,其对HFL细胞促增殖的作用越明显。结论:IL-1β可能参与调节肺成纤维细胞合成和分泌MMP-2及细胞的增殖,从而促进肺癌的侵袭和转移。Objective：To evaluate the influence of IL-1β on the expression of MMP-2 of HFL cells in three-dimensional culture collagen gels.Methods：HFL cells were cultured in three-dimensional culture collagen gels.HFL cells were randomly divided into control and experimental groups.Cells of the control group were cultured in DMEM media without IL-1β.Cells of experimental groups were cultured in DMEM media with different concentrations of IL-1β（10,20 ng/ml）,respectively.The phase contrast microscope was used to observe the morphology of cells.After 24,72 hours,harvested the supernatant,MMP-2 were detected by gelatin zymography.The proliferation of HFL cell stimulated by IL-1 β was examined by tetrazolium salt（MTT）assay.Results：In three-dimensional co-culture,HFL cells were growing in gel-culture,and it didn＇ t proliferate,and all of the cells were growing in singles.The expression of MMP-2 was different in different IL-1β concentration,and the stimulus was enhanced with the increasing of IL-1β doses.IL-1β can enhance HFL proliferation in a concentration-dependent Manner.The different concentrations of IL-1β had different effects on proliferation of HFL（P0.05）,the proliferation ability was dose-time dependent.Conclusion：IL-1β may enhance the invasion and metastasis of lung cancer throughup-regulating the secretion and of MMP-2,and enhance HFL proliferation.

关 键 词：人肺成纤维细胞 MMP-2 侵袭 转移 增殖 

分 类 号：R73-362[医药卫生—肿瘤] R734.2[医药卫生—临床医学]