急性髓细胞白血病儿童Wilms瘤基因1及其剪接异构体的表达及临床意义  

Expressions of Wilms tumor gene 1 and isoform WT1(17AA+) in children with acute myeloid leukemia and their clinical significance

作　　者：王颖[1] 胡绍燕[1] 满孝蕊[1] 吴水燕[1] 岑建农[2] 李捷[1] 何海龙[1] 陈子兴[2] 

机构地区：[1]苏州大学附属儿童医院血液肿瘤科,苏州215003 [2]苏州大学附属第一医院,江苏省血液研究所血液科,苏州215006

出　　处：《肿瘤》2011年第6期527-531,共5页Tumor

基　　金：江苏省自然科学基金资助项目(编号:BK2009127);江苏省高校自然科学研究计划资助项目(编号:06KJB320101);江苏省卫生厅科技项目(编号:H200921)

摘　　要：目的:探讨急性髓细胞白血病(acute myeloblastic leukemia,AML)患儿骨髓细胞中Wilms瘤基因1(Wilms tumor gene1,WT1)及其剪接异构体WT1(17AA+)的表达及临床意义。方法:应用实时荧光定量PCR(real-time fluorescence quantitative-PCR,RFQ-PCR)检测112例次AML患儿不同阶段骨髓细胞中WT1和WT1(17AA+)mRNA的相对表达量,计算WT1(17AA+)/WT1的比值,并以同期30例非白血病患儿作为对照。结果:AML初诊组患儿的WT1和WT1(17AA+)mRNA相对表达量均明显高于非白血病对照组及缓解期患儿(P<0.05)。复发组患儿的WT1和WT1(17AA+)mRNA相对表达量与初诊组和耐药组相比,差异无统计学意义(P>0.05)。缓解组患儿的WT1(17AA+)/WT1比值明显低于初诊组、复发组和耐药组(P<0.05)。3例AML患儿的动态监测结果显示,临床耐药或复发患儿的WT1和WT1(17AA+)mRNA相对表达量以及WT1(17AA+)/WT1比值均呈持续高表达,或者表现为一过性下降后的再度上升。结论:WT1及WT1(17AA+)mRNA剪接异构体可能成为判断AML预后及临床治疗疗效的指标。Objective：To explore the expressions of Wilms tumor gene 1（WT1） and isoform WT1（17AA＋） in bone marrow cells from children with acute myeloid leukemia（AML） and their clinical significance.Methods：The relative expression levels of WT1 and WT1（17AA＋） mRNAs in bone marrow cells from 112 cases of AML in different disease courses were detected by real-time fluorescence quantitative-PCR.The ratio of WT1（17AA＋）/WT1 was calculated.Thirty non-leukemia children were recruited as the controls.Results：The relative expression levels of WT1 and WT1（17AA＋） mRNAs in newly diagnosed AML children were significantly higher than those in the complete remission（CR）-phase group and the control group（P〈0.05）,while there was no difference among the relapsed,newly diagnosed and drug-resistant groups（P〉0.05）.The ratio of WT1（17AA＋）/WT1 in CR-phase group was significantly lower than those in the newly diagnosed,relapsed and drug-resistant groups（P〈0.05）.The dynamic monitoring of three children with AML revealed that there was a tendency that the relative expression levels of WT1 and WT1（17AA＋） and the ratio of WT1（17AA＋）/WT1 were high or increased again after a transient decrease.Conclusion：WT1 and WT1（17AA＋） may predict the prognosis and therapeutic efficiency for AML.

关 键 词：白血病 髓样 儿童 Wilms瘤基因1 实时荧光定量PCR 

分 类 号：R733.7[医药卫生—肿瘤]