慢性牙周炎合并动脉粥样硬化兔的血管内皮功能与升主动脉E选择素的表达  

Vascular endothelial function and E-selectin expression in ascending aorta in rabbits with chronic periodontitis and atherosclerosis

作　　者：高云[1] 钟良军[2] 张源明[1] 周晓欢[1] 梁平[1] 徐隽[2] 

机构地区：[1]新疆医科大学第一附属医院心脏中心,乌鲁木齐830054 [2]新疆医科大学口腔医学系

出　　处：《中华高血压杂志》2011年第6期544-548,共5页Chinese Journal of Hypertension

基　　金：国家自然科学基金(30960135);自治区科技攻关和重点项目计划(200733146-3)

摘　　要：目的应用新西兰大白兔建立慢性牙周炎(CP)合并动脉粥样硬化(AS)的动物模型,探讨CP对AS血管内皮功能的影响。方法 将32只雄性新西兰大白兔随机分为4组:对照组、CP组、AS组、CP+AS组。各组分别按照实验设计进行相应干预处理,至14周末处死动物,检测血清中血脂、内皮素1、一氧化氮含量,观察实验牙X线片及升主动脉的病理改变,测定主动脉壁E选择素表达的阳性面积百分比。结果 高脂喂养升高血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平(P<0.01),牙周炎升高血清三酰甘油水平(P<0.05)。对照组、CP组、AS组、CP+AS组血清内皮素1水平分别为(87.7±10.1)、(104.9±8.9)、(112.7±14.3)、(132.2±10.5)ng/L,血清一氧化氮水平分别为(31.83±4.21)、(28.34±4.29)、(27.75±2.94)、(25.59±3.03)μmol/L;析因设计资料方差分析表明,CP和AS均可升高血清内皮素1和升主动脉壁E选择素的表达水平,降低血清一氧化氮水平(均P<0.05),两种疾病合并未见明显协同效应(P>0.05)。结论 CP和AS均可导致血管内皮功能不全,两种疾病合并未见协同作用。Objective To investigate the influence of chronic periodontitis （CP） on the endothelial function of atherosclerosis （AS） by establishing animal models of CP and AS in rabbits. Methods Thirty-two male New Zealand rabbits were divided into four groups at random： control group, CP group, AS group and CP＋AS group. Each group received corresponding treatment. Animals were killed after 14 weeks. Levels of blood lipids, endothelin-1 （ET-1） , and nitric oxide （NO） were examined. Periodontitis severity was observed radiographically, while ascending aorta surveyed histologically. The percentage of the positive area of E-selectin in ascending aorta wall investigated by immunohistochemistry method. Results High-fat diet increased the levels of serum total cholesterol, triglyceride, high-density lipoprotein cholesterol （HDL-C） and low-density lipoprotein cholesterol （LDL-C） （P〈0. 011, while CP increased serum triglyceride level （P〈0.05）. In the control group, CP group, AS group and CP＋ AS group, serum ET 1 levels were （ 87.7±10.1 ） , { 104.9 ± 8.9 }, { 112.7 ± 14.3） and { 132.2 ± 10.5 ） ng/L, while serum NO levels were （31.83±4.21）, （28.34±4.29）, （27.75±2.94） and （25.59±3.03）μmol/L, respec tively. Analysis of variance of factorial design data showed that CP and AS upregulated serum ET-1 level and the expresssion of E-selectin in ascending aorta wail, but downregulated serum NO level （all P〈0.05）. There was no synergetic or additive effect between AS and CP in relation to the above indexes（P〉0.05）. Conclusion Both CP and AS can lead to vascular endothelial dysfunction, and no synergetic effect occurs between the two diseases.

关 键 词：牙周炎 动脉粥样硬化 动物模型 内皮功能 E选择素 

分 类 号：R781.4[医药卫生—口腔医学] R543.5[医药卫生—临床医学]