p53基因和FHIT基因联合检测在肺癌早期诊断中的意义  

Significance of p53 Gene and FHIT Gene Union Examination in Early Diagnosis of Lung Cancer

作　　者：王盛超[1] 王红岩[1] 阎慧娟[1] 马芸[2] 

机构地区：[1]河南科技大学第一附属医院,河南洛阳471003 [2]河南省人民医院,河南郑州450000

出　　处：《河南科技大学学报（医学版）》2011年第2期92-96,共5页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的探讨p53基因突变和FHIT基因缺失在肺癌的筛查和早期诊断中的应用价值。方法收集利用自荧光支气管镜(AFB)活检的27例正常支气管黏膜、74例非典型增生、23例原位癌、161例浸润癌及32例良性病变新鲜标本,应用聚合酶链式反应(PCR)及DNA测序技术检测p53基因突变,应用逆转录聚合酶链式反应(RT-PCR)及DNA测序技术检测FHIT基因表达缺失。结果在正常黏膜、非典型增生、原位癌、浸润癌及良性病变中p53基因突变率分别为:0、27.0%、52.2%、67.7%、0。FHIT基因缺失率分别为:0、55.4%、65.2%、66.5%、9.4%。两基因联合检测阳性率分别为:0、56.8%、86.9%、90.7%、9.4%。非典型增生与原位癌组织中p53基因突变差异有统计学意义(P<0.05),非典型增生、原位癌、浸润癌组织FHIT基因缺失差异无统计学意义(P>0.05),组织病理学分类不同的浸润癌组织p53基因突变率和FHIT基因缺失率差异有统计学意义(P<0.05)。结论 p53基因突变和FHIT基因缺失在肺癌及非典型增生组织中是频发事件,在肺癌的发生发展中起重要作用,两基因联合检测可提高肺癌诊断的敏感性及特异性,对肺癌的早期诊断有重要意义。Objective To research the normal bronchial mucosa,atypical hyperplasia,carcinoma in situ,infiltration carcinoma and benign lesions the p53 gene mutation and FHIT gene deletion and to investigate in lung cancer screening and early diagnosis value. Methods To collect and use fluorescent bronchoscopy biopsy of 27 normal bronchial mucosa,74 atypical hyperplasia cases,23 carcinomas in situ,161 infiltrating carcinoma cases and 32 benign lesions fresh specimens cases,application polymerase chain reaction（PCR） and DNA sequencing technologies detection p53 gene mutation,application retrovirus polymerase chain reaction（PCR） and RT-DNA sequencing technologies detection FHIT gene expression missing. Results p53 gene in normal bronchial mucosa,atypical hyperplasia,carcinoma in situ,infiltration carcinoma and benign lesions,the mutation rate were 0,27.0%,52.2%,67.7%,0%,respectively.The FHIT gene expression missing rate were 0,55.4%,65.2%,70.8%,9.4%,respectively.The positive rate of combined detection of the two genes were： 0,56.8%,86.9%,90.7%,9.4%.Atypical hyperplasia and carcinoma in situ tissue p53 gene mutation was significant（P0.05）,Atypical hyperplasia,carcinoma in situ,infiltration carcinoma tissue FHIT gene deletion was not significant difference（P0.05）,the organization pathology classification different infiltration cancer organizes the p53 gene mutation and the FHIT gene deletion difference had statistic significance（P0.05）. Conclusion p53 gene mutation and FHIT gene deletion in lung cancer and atypical hyperplasia is a frequent event in the development of lung cancer which plays an important role,Two gene union examination may enhance the lung cancer diagnosis the sensitivity and the specificity,has the important meaning to early diagnosis of the lung cancer.

关 键 词：肺癌 P53基因 FHIT基因 支气管镜 荧光 早期诊断 

分 类 号：R734.2[医药卫生—肿瘤]