褪黑素对脂多糖引起胎鼠脑细胞钙超载损伤的神经保护作用  

The neuroprotective effect of melatonin on calcium overload-associated injury of fetal mouse brain cells after injection of lipopolysaccharide

作　　者：贾玉芳[1,2] 钱志红[1] 柴月荣[1] 崔玉[1] 

机构地区：[1]苏州大学附属第二医院妇产科,215004 [2]溧阳市人民医院妇产科

出　　处：《江苏医药》2011年第13期1523-1525,共3页Jiangsu Medical Journal

摘　　要：目的探讨褪黑素(MT)对脑损伤的保护作用。方法 72只妊娠第19天SD孕鼠随机均分为三组:A组,腹腔注射细菌脂多糖(LPS)500μg/kg;B组,腹腔注射LPS 500μg/kg+MT10 mg/kg;C组,腹腔注射等容积生理盐水。分别于给药后1、6、12、24、48、72 h剖腹取出胎鼠,急性分离脑组织,新型荧光探针Fluo-3/AM负载胎鼠脑细胞,流式细胞术检测胎鼠细胞内游离钙的平均荧光强度。结果注药后,A、B组脑细胞钙离子荧光值于6 h开始明显上升,12 h达高峰,各观测时点的钙离子荧光值均明显高于C组(P<0.01)。除72 h外,B组其他各观测时点的钙离子荧光值均明显低于A组(P<0.01或P<0.05)。结论 LPS可诱导胎鼠脑细胞内钙离子超载,导致脑损伤。MT通过抑制细胞内钙超载对脑细胞损伤有保护作用。Objective To investigate the neuroprotective effect of melatonin on calcium overload-associated injury of fetal mouse brain cells after injection of lipopolysaccharide（LPS）.Methods A total of 72 SD rats with 19 days of pregnancy was equally randomized into three groups of A（treated with intraperitoneal injectio of LPS 500μg/kg）,B（treated withintraperitoneal injection of LPS 500μg/kg plus MT 10 mg/kg） and C（injected normal saline as blank control）.The fetuses were taken out at the 1st,6th,12th,24th,48th,or 72ndh after injection,respectively,for detecting the fluorescence intensity of intracellular calcium of fetal brain tissues by flow cytometry after novel fluorescent probe Fluo-3AM loading.Results Compared to before,the calcium fluorescence values of brain cells were significantly increased on th 6thh and peaked on th 12thh after drug injection（P0.01）.The calcium fluorescence values of brain cells were all significantly higher in groups of A and B than thoae in group C at all time points after injection（P0.01）,which were lower in group B than those in group A at all time points except for the 72ndh（P0.01 or P0.05）.ConclusionLPS results in brain damage by inducing intracellular calcium overload in brain cells of fetal rat,which may be attenuated by MT injection through inhibiting intracellular calcium overload.

关 键 词：褪黑素 脂多糖 钙超载 

分 类 号：R363[医药卫生—病理学]