尖山橙和龙州山橙中的抗肿瘤吲哚生物碱(英文)  

Cytotoxic Indole Alkaloids from Melodinus fusiformis and M.morsei

作　　者：蔡祥海[1] 蒋皓[1] 李艳[1] 程桂广[1] 刘亚平[1] 冯涛[1] 罗晓东[1] 

机构地区：[1]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650204

出　　处：《中国天然药物》2011年第4期259-263,共5页

基　　金：supported by the National Natural Science Foundation of China(No.21072198);the West Light Foundaton of the Chinese Academy of Sciences(No.KSCX2-YW-EW-15)~~

摘　　要：目的:研究尖山橙和龙州山橙中的抗肿瘤生物碱成分方法:采用层析柱和制备高效液相进行化合物分离,运用1D、2D-NMR及MS等波谱分析技术鉴定化合物结构。采用MTT法进行人体肿瘤细胞毒体外活性实验。结果:分离得到两个新的双吲哚生物碱melofusine I (1)和melomorsine I (2),以及22个已知吲哚生物碱,测试了24个化合物对5种(SW480,SMMC-7721,HL-60,MCF-7,A-549)人体肿瘤细胞的细胞毒活性。结论:化合物1-2是新的双吲哚生物碱,化合物tabersonine(3) and 19S-vindolinine(14)具有抑制5种人体肿瘤细胞株的活性。AIM：To investigate antitumor indole alkaloids from the leaves and twigs of Melodinus fusiformis and M.morsei.METHODS：The indole alkaloids were isolated by column chromatography and preparative HPLC.Their structures were elucidated by 1D and 2D-NMR spectroscopic methods in combination with MS experiments.Their cytotoxicity were evaluated using the MTT method in 96-well microplates.RESULTS：Two new bisindole alkaloids,melofusine I（1） and melomorsine I（2）,together with 22 known indole alkaloids 3-24,were isolated from M.fusiformis and M.morsei.All isolated compounds were tested against five human tumor cell lines,SW480,SMMC-7721,HL-60,MCF-7 and A-549.CONCLUSION：Compounds 1 and 2 are new bisindole alkaloids,whereas,compounds tabersonine（3） and 19S-vindolinine（14） showed significant cytotoxicity against the above-mentioned five human tumor cell lines.

关 键 词：尖山橙 龙州山橙 Melofusine I Melomorsine I 双吲哚生物碱 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]