实时荧光定量检测肝细胞肝癌SATB1 mRNA的表达及其临床意义  

Expression of special AT-rich sequence-binding protein 1 mRNA in hepatocellular carcinoma and its clinical significance

作　　者：彭正奎[1,2] 杨定华[1] 李湘竑[1] 黄毓[3] 张国强[1] 钟克波[1] 毕民平[1] 李光辉[1] 

机构地区：[1]南方医科大学南方医院肝胆外科,广东广州510515 [2]兰州大学第二医院普外科,甘肃兰州730030 [3]南方医科大学南方医院检验科,广东广州510515

出　　处：《南方医科大学学报》2011年第7期1207-1211,1215,共6页Journal of Southern Medical University

基　　金：广东省自然科学基金面上项目(C1030281)

摘　　要：目的探讨肝癌中核基质结合区结合蛋白-1的表达及其临床意义。方法用TRIZOL提取102例肝癌组织及对应的癌旁组织,并将其逆转录为cDNA。用实时荧光定量PCR方法检测肝细胞肝癌中SATB1mRNA的表达,并分析SATB1基因的表达程度与患者临床病理参数、术后复发与转移的关系。结果肝细胞肝癌组织中SATB1的表达是癌旁组织的3.27倍(P<0.001),有统计学差异。癌组织中SATB1mRNA表达的高低与肝硬化、AFP、肿瘤大小、癌栓、病理分化、TNM分级、术后复发转移有关系(P<0.05)。与性别、年龄、乙型肝炎表面抗原、丙型肝炎抗体、肿瘤的数目、包膜无关(P>0.05)。SATB1mRNA显著高表达组、高表达组、低表达组的复发率分别为82.68%,0,0;而三组的一年累计生存率分别为0,52.63%,100%。结论 SATB1mRNA的表达与肝癌的复发转移有关,SATB1有望成为肝癌复发与转移的一个新的预后指标。Objective To investigate the expression of special AT-rich sequence binding protein 1（SATB1） mRNA in hepatocellular carcinoma（HCC） and explore its correlation to the clinicopathological features,surgical outcomes and metastasis of HCC.Methods The total RNA was extracted from 102 HCC tissues and the adjacent tissues,and the expression of SATB1 mRNA was detected using quantitative real-time PCR.The correlations of SATB1 mRNA expression to the clinicopathological features,postoperative recurrence and metastasis of the tumor were analyzed.Results The expression of SATB1 mRNA in HCC tissues was 3.27 folds higher than that in the adjacent tissues（P〈 0.001）.The expression of SATB1 mRNA in HCC was associated with liver cirrhosis,AFP level,tumor size,tumor thrombi,histological differentiation,TNM classification,postoperative recurrence and metastasis（P〈0.05）,but not to the patients’ gender,age,HbsAg positivity,HCV-Ab positivity,tumor number,or the presence of tumor encapsulation（P〉0.05）.In patients with significant high expression,high expression,and low expression of SATB1 mRNA,the postoperative recurrence rates were 82.68%,0,and 0,with the 3-year survival rate of 0,52.63%,and 100%,respectively.Conclusion SATB1 mRNA expression is associated with the postoperative recurrence and metastasis of HCC,and can be used as an indicator for predicting the recurrence and metastasis of HCC.

关 键 词：肝细胞肝癌 核基质结合区结合蛋白-1信使RNA 实时荧光定量RT-PCR 

分 类 号：R735.7[医药卫生—肿瘤]