妊娠期糖尿病合并妊娠期高血压疾病血清差异蛋白的鉴定  

Identification of protein markers for gestational diabetes mellitus complicated by pregnancy-induced hypertensive syndrome

作　　者：王硕石[1] 胡水旺[2] 钟梅[1] 

机构地区：[1]南方医科大学南方医院妇产科,广东广州510515 [2]南方医科大学病理生理学教研室,广东省蛋白质组学重点实验室,广东广州510515

出　　处：《南方医科大学学报》2011年第7期1224-1227,共4页Journal of Southern Medical University

基　　金：广东省科技计划资助项目(2007B030502015)

摘　　要：目的寻找妊娠期糖尿病合并妊娠期高血压疾病的分子标记物,为筛查及防治该类疾病提供分子生物学基础。方法收集妊娠期高血压疾病、妊娠期糖尿病、妊娠期糖尿病合并妊娠期高血压疾病患者的静脉血清标本各4份,去除高丰度的免疫球蛋白和白蛋白。应用十二烷基磺酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)技术将可溶性血清总蛋白分离。切取在不同样品中显示明显差异的蛋白条带,酶解后使用质谱技术对所得的差异蛋白进行鉴别。运用生物信息学分析差异蛋白的生物学功能。结果将上述去除高丰度蛋白的标本进行3次重复的SDS-PAGE,确定了显示显著差异的蛋白条带。质谱分析结果表明这些差异性蛋白分别为:结合珠蛋白、SMG8蛋白和凋亡诱导因子-1。结论为建立妊娠期代谢性疾病的蛋白质数据库提供了基础性数据。鉴定了与该疾病显著相关的部分蛋白,为进一步预防该疾病、改善妊娠结局以及阐明该疾病的分子机制提供了可参考的实验依据。Objective To identify the serum protein markers for the gestational diabetes mellitus（GDM） complicated by pregnancy-induced hypertensive（PIH） syndrome to provide a molecular biological basis for the screening,prevention and therapy of the related diseases.Methods Serum samples were collected from the patients with GDM,PIH syndrome,and GDM complicated by PIH syndrome.IgG and albumins were removed from the samples before SDS-PAGE.The protein bands showing significant differences among the 3 samples were collected,digested and identified with mass spectrometry,and the function of the identified proteins was analyzed.Results Three SDS-PAGE were performed in parallel to confirm the differentially expressed proteins.Mass spectrometry indicated that the proteins showing obvious differences among the 3 samples were haptoglobin,protein SMG8 and apoptosis-inducing factor-1.Conclusions The protein markers identified in GDM complicated by PIH syndrome may be integrated into the proteomic database of gestational metabolic diseases.Identification of the associated protein markers may provide significant experimental data for the prevention,diagnosis and therapy of the related diseases.

关 键 词：妊娠期糖尿病合并妊娠期高血压疾病 妊娠期高血压疾病 妊娠期糖尿病 差异蛋白 质谱 

分 类 号：R714.2[医药卫生—妇产科学]