融合蛋白CAC免疫对大鼠海马内注射β-淀粉样肽的作用  

Immunization with recombinant HBcAg and β-amyloid peptide fusion protein promotes clearance of intrahippocampally injected β-amyloid peptide in rats

作　　者：冯改丰[1] 胡海涛[1] 靳辉[1] 王唯析[1] 钱亦华[1] 

机构地区：[1]西安交通大学医学院人体解剖与组织胚胎学系,陕西西安710061

出　　处：《南方医科大学学报》2011年第7期1236-1239,共4页Journal of Southern Medical University

基　　金：陕西省自然科学基金(2001SM57);西安交通大学科学研究基金(2002003)

摘　　要：目的观察融合蛋白CAC(HBcAg与β-淀粉样肽基因重组的原核表达产物)免疫对大鼠海马内注射聚集态β-淀粉样肽(Aβ)的作用。方法用融合蛋白CAC免疫SD大鼠,通过ELISA方法检测其血清中的Aβ抗体的滴度。当滴度大于1:3000时,给大鼠海马CA1区注射聚集态Aβ1-40。2周后进行行为学测试,病理学检测。结果融合蛋白免疫5次后,大鼠血清中Aβ抗体的滴度达到了1:3000。未经CAC免疫的大鼠,在海马内注射Aβ后,学习记忆能力显著减低,注射区可见细胞损伤和刚果红染色的淀粉样沉积,伴有大量的胶质细胞浸润。CAC免疫大鼠的学习记忆能力有明显改善,淀粉样沉积面积减少或几乎消失。结论融合蛋白CAC免疫可抑制海马内注射Aβ的毒性作用,促进其降解和排出。Objective To study the effects of immunization with the fusion protein CAC（a product of prokaryotic expression of recombinant HBcAg and β-amyloid peptide fusion gene） against the toxicity induced by intrahippocampal injection of aggregated β-amyloid peptide（Aβ） in rats.Methods SD rats were immunized intraperitoneally with the fusion protein CAC,and the titer of anti-Aβ antibody was evaluated by ELISA.When the titers of the anti-Aβ antibody reached 1：3 000,aggregated Aβ was injected into the CA1 region of the rat hippocampus.Two weeks after Aβ injection,the rats underwent morris water maze test before sacrificed to prepare the brain slices with Congo red and haematoxylin staining.Results The titer of anti-Aβ antibody reached 1：3 000 after 5 immunizations with the fusion protein.After Aβ injection,the saline-immunized rats showed a reduced cognitive behavior in the Morris water maze test compared to the CAC-immunized rats.In the saline-immunized rats,the neurons around the site of Aβ injection exhibited obvious cell damages with Aβ deposits and glial infiltration,whereas in CAC-immunized rats,Aβ deposits were significantly reduced or even absent.Conclusion Immunization with the fusion protein CAC can inhibit the toxicity induced by intrahippocampal aggregated Aβ injection.

关 键 词：融合蛋白CAC Β-淀粉样肽 海马 免疫 

分 类 号：R749.16[医药卫生—神经病学与精神病学]