小鼠肝炎病毒温病湿热证模型的探讨  

To Approach Mouse Models of Damp-heat Syndrome of Seasonal Febrile Disease Infected by MHV-A59

作　　者：李华锋[1] 林培政[2] 

机构地区：[1]广州医学院第二附属医院中医科,广东广州510260 [2]广州中医药大学温病学教研室,广东广州510405

出　　处：《辽宁中医杂志》2011年第7期1318-1321,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金广东省自然科学基金联合项目(U0632009)

摘　　要：目的:研究拟以MHV-A59和大肠杆菌作为生物致病因子,建立两种小鼠温病湿热证动物模型,通过对比探讨小鼠肝炎病毒温病湿热证模型可行性。方法:动物随机分为3组:正常组、模型组(肥甘饮食+湿热外环境+MHV-A59造模)、肠菌组(肥甘饮食+湿热外环境+大肠杆菌造模),检测小鼠血清TG、TNF-α水平及肝组织病理切片,观察动物症状及体征。结果:与正常组比较,两种温病湿热证模型组小鼠血清TG及TNF-α水平明显升高(P<0.01),两组造模动物均不同程度出现了发热、疲倦、纳呆、腹泻、体重减少、毛发无泽等症状。结论:以MHV-A59作为生物致病因子,结合肥甘饮食及湿热外环境因素构建的动物模型,可以作为温病湿热证动物模型之一,也是一种新的病证结合模型。Objective：Through contrast mouse models of Damp-heat syndrome of seasonal febrile disease infected by MHV-A59 and Bacillus coli.To establish Mouse Models of Damp-heat Syndrome of Seasonal Febrile Disease Infected by MHV-A59.Methods：four-weeks-old male mouse of BALB/c was divided into three groups randomly,control group,model group（richly fatty and sweet diet ＋ humid heat environment ＋ infecting by MHV-A59）,Bacillus coli group（richly fatty and sweet diet ＋ humid heat environment ＋ infecting by Bacillus coli）.The TG and TNF-αlevel in serum of mouse were detected by biochemistry and ELISA.In experiment we observed the symptom and sign of mouse.Results：The level of TG and TNF-αin serum of two model group obviously risen up compared to control group（P0.01）.The mouse of two model group show up fever,languor,Diarrhea,weight lose,poor appetite etc.Conclusion：we duplicated a new mouse model of damp-heat syndrome of seasonal febrile disease by richly fatty and sweet diet,humid heat environment,MHV-A59.The model possessed characteristics of the illness and syndrome.

关 键 词：温病湿热证 动物模型 MHV-A59 TG TNF-Α 

分 类 号：R-332[医药卫生]