超声波提取硬头黄竹叶黄酮工艺优化  

Optimization of Ultrasonic Extraction Process for Flavonoids from Bambusa Rigida Leaves

作　　者：林娜[1,2] 尹礼国[1] 陈云珠[1] 

机构地区：[1]宜宾学院生命科学与食品工程学院,四川宜宾644000 [2]发酵资源与应用四川省高校重点实验室,四川宜宾644000

出　　处：《安徽农业科学》2011年第18期10816-10817,10819,共3页Journal of Anhui Agricultural Sciences

基　　金：发酵资源与应用四川省高校重点实验室基金项目(2010KF-J002);宜宾市科技局重点项目(2010NY005);宜宾市科技局重点项目(200801007)

摘　　要：[目的]优化超声波提取硬头黄竹(Bambusa rigida)叶黄酮的工艺。[方法]通过单因素试验研究不同溶剂、乙醇浓度、提取温度、料液比和提取时间对提取效果的影响,通过正交试验对提取工艺进行优化。[结果]在超声波功率为600 W时,各因素对黄酮提取量的影响顺序为:乙醇浓度>提取温度>料液比>提取时间。最佳的提取工艺为:乙醇浓度为85%,提取温度为75℃,提取时间90 min、料液比1∶30,在该条件下黄酮提取量为11.78 mg/g。[结论]该研究得到了硬头黄竹叶黄酮提取的最佳工艺,为竹叶黄酮工业化生产工艺的建立提供了依据。[ Objective ] To optimize the ultrasonic extraction process for extracting flavonoids from Bambusa rigida leaves. [ Method ] Effect of solvent kind,alcohol concentration,extraction temperature, solid to liquid ratio and extraction time on extraction efficiency of flavonoids were in- vestigated through single factor experiments and the extraction technology was optimized by orthogonal test. [ Result] The factors influencing ex- traction of flavonoids with ultrasonic power at 600 W were in order as follows ： alcohol concentration 〉 extraction temperature 〉 ratio of solid-to- liquid ratio 〉 extraction time. The optimal extraction conditions were：alcohol concentration of 85%, extraction temperature of 75 ℃, extraction time of 90 min and solid to liquid ratio of 1 ： 30. Under this condition, the content of flavonoids from Bambusa rigida leaves was 11.78 mg/g. [ Conclusion] The optimal technology for extracting flavonoids from Bambusa rigida leaves had obtained in this work, which provided references for industrial production of bamboo leaf flavonoids.

关 键 词：硬头黄竹(Bambusa rigida) 竹叶 黄酮 超声波 提取工艺 

分 类 号：S567.9[农业科学—中草药栽培]