绿原酸水解产物的高效液相色谱—电喷雾串联质谱分析  

HPLC-ESS/MS analysis of chlorogenic acid and its hydrolysate

作　　者：罗奇志[1] 王有志[2] 罗佳波[2] 

机构地区：[1]南方医科大学肿瘤研究所 [2]南方医科大学中医药学院,广州510515

出　　处：《药物分析杂志》2011年第7期1345-1349,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:分析双黄连方药制备过程中影响绿原酸水解的因素及可能的水解产物。方法:采用高效液相色谱-电喷雾串联质谱法分析绿原酸在不同pH值条件及不同水解时间的水解产物。结果:绿原酸在pH6.85的水溶液中较稳定,在酸性条件下比碱性条件下稳定。结论:绿原酸的水解模式主要有分子间酯键断裂、咖啡酰基迁移、水合反应等。Objective：To investigate the main factors that induce the chlorogenic acid hydrolyse and the related hydrolysate during the preparation of Shuang-huang-lian decotion.Methods：HPLC-ESI-MSn were used to indentify the hydrolysate of chlorogenic acid in different conditions.Results：chlorogenic acid was stable in the water with pH value 6.85,it was more stable in the acidic water than in the basic water.Conclusion：the hydrolyse of chlorogenic acid was supposed as cleavage of the ester bond,cleavage of the ester bond with a rearrangement of the caffeoyl residue or the addition of a molecule of water.

关 键 词：绿原酸 水解 液相色谱 质谱 双黄连 中药 金银花 

分 类 号：R917[医药卫生—药物分析学]