雌激素与孕激素对卵巢癌耐药细胞系OVCAR-3生长及耐药的影响  

Effects of Estradiol and Medroxyprogesterone on the Growth and Doxorubinresistance of Drug-resistant Human Epithelial Ovarian Cancer Cell the OVCAR-3

作　　者：孟君艳[1] 尤宗兵[1] 郭燕燕[1] 

机构地区：[1]北京医科大学第一医院妇产科,航天部七一一医院妇产科100034

出　　处：《中华妇产科杂志》1999年第11期670-673,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：卫生部基金!96-1-280

摘　　要：目的 研究雌、孕激素对卵巢癌耐药细胞系ＯＶＣＡＲ－３生长及对阿霉素（ＡＤＭ）耐药的影响。方法 用四甲基偶氮唑蓝（ＭＴＴ）快速比色法，计算细胞生存率，以比较不同浓度雌、孕激素对细胞生长的影响，及雌，孕激素与异搏定对卵巢癌耐药细胞系ＯＶＣＡＲ－３耐药性的影响。Objective To study the effects of estradiol (E2) and medroxyprogesterone acetate (MPA) on the growth of drug resistant human epithelial ovarian cancer cell line. Methods OVCAR-3 cells, treated by different concentrations of E2, MPA and verapamil, were examined for their growth inhiitions with methyl thiazlyl tetrazolium (MTT, a tetrazolium dye) rapid photocolorimetric assay. Results OVCAR-3 cell growth was inhibted by E2 at high concentrations (10.00- 100.00 μmol/L)and by MPA at 100.00μmol/L, while lower concentrations of E2(0.01-1 .00 μmol/L) and MPA(0.01-10.00 μmol/L) had no effect on OVCAR-3 cell growth, neither stimulation nor inhibihon. The effect of E2 combined with MPA was more Obvious than either one used separately.E2 and MPA in combination with doxorubin showed a synergistic inhibition effect on OVCAR-3 cell growth, which suggesed that both of them could reverse the doxorubin resistance of OVCAR-3 cell line. The inhidition effect of Verapamil combined with doxorubin was obviously weaker than that of E2 and MPA. Conclusions E2 and MPA could reverse the doxorubin resistance of OVCAR-3 cell line and their inhibhon were more obvious than the of verapamil, which Suggested that E2 and MPA may be used clinically for adjuvant chemotherapy.

关 键 词：雌激素 卵巢肿瘤 孕激素 OVCAR-3 耐药性 

分 类 号：R737.31[医药卫生—肿瘤]