中华鳖病原维氏气单胞菌的分子鉴定及分子分类学研究  

Molecular Identification of Aeromonas veronii Isolated from Soft-shelled Turtles and Phylogenetics Analysis of Aeromonas(Aeromonadaceae)

作　　者：赵宇亮[1] 吕梦溪[1] 苏欣[1] 钱明明[1] 武斌[1] 赵宝华[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016

出　　处：《河北师范大学学报（自然科学版）》2011年第4期417-422,共6页Journal of Hebei Normal University：Natural Science

基　　金：河北省重点科技攻关项目(06780503);河北师范大学科技创新基金(AD2010327162550)

摘　　要：气单胞菌是一类人和水产经济动物的严重致病菌,用分子进化手段鉴定一株分离自红脖子病甲鱼病灶部位的菌HBJY01,同时探讨气单胞菌的进化地位.克隆HBJY01的16S rRNA和gyrB基因,鉴定该菌的属种.用16S rRNA和gyrB基因通过构建系统进化树和计算序列两两间遗传距离的方法,探讨气单胞菌的进化地位.HBJY01的16S rRNA和gyrB基因与维氏气单胞菌的相应基因同源性最高,经分子鉴定为维氏气单胞菌.气单胞菌应独立于弧菌科,单独形成一个科,即气单胞菌科(Aeromonadaceae).Aeromonas is a kind of bacteria which could infect human and aquatic animals.One Aeromonas strain of pathogenic bacteria HBJY01,isolated from diseased Trionyx sinensis,was identified through molecular method.Phylogenetics status of Aeromonas（Aeromonadaceae） was analyzed.Using the 16S rRNA and gyrB genes,the Aeromonas strain was identified,bacterial phylogenetics trees were constructed and pairwise distance was calculated.Based on these,phylogenetics status of Aeromonas（Aeromonadaceae） was analyzed.HBJY01 was very similar to Aeromonas veronii in terms of 16S rRNA and gyrB gene sequences.In the genetic distance,Aeromonas and other Vibrionaceae had great differences.HBJY01 was identified as Aeromonas veronii.Aeromonas should be an independent new family,Aeromonadaceae,not be a genus of Vibrionaceae.

关 键 词：维氏气单胞菌 16SRRNA基因 gyrB基因 系统发育分析 

分 类 号：S945[农业科学—水产养殖]