人纤溶酶原kringle5功能区基因在大肠杆菌中的表达  

Expression of Kringle 5 of Human Plasminogen in E coli

作　　者：陈广南[1] 罗进贤[1] 卢文菊 张添元[1] 

机构地区：[1]中山大学生物化学系/生物工程研究中心/生物防治国家重点实验室,广州510275

出　　处：《中山大学学报（自然科学版）》1999年第6期62-66,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金! ( 3 9670 0 1 3 );广东省科学基金! ( 960 0 5 2 )

摘　　要：将人纤溶酶原kringle5功能区基因插入融合基因表达载体pET17bBamHⅠ位点,构建成重组质粒pETK23,转化大肠杆菌BL21(DE3),在IPTG诱导下,纤溶酶原kringle5功能区基因在重组转化菌株BL21(DE3,pETK23)中获得高效表达,表达产物以可溶性蛋白形式存在,表达量占菌体总蛋白的528%.Kringle 5 of human plasminogen is a recently discovered inhibitor of endothelial cell growth and migration It may also supress angiogenesis and tumor growth The gene encoding kringle 5 of human plasminogen was cloned in the Bam HⅠsite of pET 17b giving rise to the plasmid pETK23 which was then transformed into E coli BL21(DE3) Kringle 5 of human plasminogen was efficently expressed in recombinant strain E coli BL21(DE3,pETK23) by IPTG induction The expression product constitutes about 52 8% of the total protein and exsists in the form of soluble protein Western blot analysis demonstrates that it reacts positively with goat anti human plasminogen antisera

关 键 词：人纤溶酶原 kringle5功能区 基因表达 大肠杆菌 

分 类 号：Q78[生物学—分子生物学] R979.1[医药卫生—药品]