农抗702及其单体组分对尖孢镰刀菌的抗菌毒力测定  

A Study on Antibiotic Toxicity of Ag-antibiotic 702 and Its Monomers to Fusarium oxysporum f.sp. cubense

作　　者：何建[1] 阮彩彪[1] 涂晓嵘[1] 涂国全[1] 

机构地区：[1]江西农业大学生物科学与工程学院,南昌市发酵应用技术重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2011年第3期493-497,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30960011、31071724);江西省自然科学基金项目(32009GZN0030、2010GZN0037);江西省科技支撑计划项目(2007BN14002)

摘　　要：探索农抗702及其两个单体组分对香蕉枯萎病菌尖孢镰刀菌古巴专化型1号和4号生理小种(FOC1、FOC4)的抗菌活性的毒力测定。分别以农抗702、其中两个单体组分Dzp8和Dzp9以及那他霉素为供试材料,以FOC1,FOC4为靶标菌。抗菌活性的测定方法采用菌丝生长速率法。那他霉素对FOC1,FOC4的EC50和EC90分别为2.08,1.71,6.6,11.96μg/mL;样品Dzp9对FOC1,FOC4的EC50和EC90分别为4.67,3.41,21.54,53.09μg/mL;农抗702对FOC1,FOC4的EC50和EC90分别为20.98,16.84,50.68,62.18μg/mL。那他霉素、Dzp9样品和农抗702这3种抗生素对供试的靶标菌有较好的抑菌作用,而供试靶标菌对Dzp8样品不敏感。Objective： To probe antibiosis activities and toxicity of agricultural antibiotic 702 and its two monomer components to Fusarium oxysporum f.sp.cubense race 1 and race 4（FOC1,FOC4）.Methods：Ag-antibiotic 702 and its＇ two monomer components Dzp8 and Dzp9 sample along with Natamycin were used as the test material,FOC1,FOC4 as the target fungi.The method for teshing the antibiosis activities was the turf growth rate method.The results were the EC 50 and EC 90 from Natamycin to FOC1,FOC4 were 2.08 mg/mL,1.71 mg/mL and 6.6 mg/mL,11.96 mg/mL respectively;the EC 50 and EC 90 from Dzp9 to FOC1,FOC4 were 4.67 mg/mL,3.41 mg/mL and 21.54 mg/mL,53.09 mg/mL respectively;the EC 50 and EC 90 from agricultural antibiotic 702 to FOC1,FOC4 were 20.98 mg/mL,16.84 mg/mL and 50.68 mg/mL,62.18 mg /mL respectively.The target fungi were sensible to Natamycin,sample Dzp9 and agricultural antibiotic 702,and they showed quite good antimicrobial activities,while the target fungi were insensitive to sample Dzp8.

关 键 词：农抗702 尖孢镰刀菌古巴专化型 抗真菌活性 毒力测定 

分 类 号：S482.2[农业科学—农药学]