细叶黄皮树茎皮乙醇提取物、挥发油的抑菌作用  

Antimicrobial Activity of Ethanol Extracts and Volatile Oil from the Bark of Clausena anisum-olens

作　　者：苏秀芳[1] 韦良兴[1] 黄丽娟[1] 冯佩珍[1] 

机构地区：[1]广西民族师范学院化学与生物工程系,广西崇左532200

出　　处：《湖北农业科学》2011年第13期2668-2670,共3页Hubei Agricultural Sciences

基　　金：广西自然科学基金项目(2010GXNSFA013050)

摘　　要：采用纸片扩散法进行抑菌试验以探讨细叶黄皮[Clausena anisum-olens（Blanco.）Merr]茎皮乙醇提取物及其石油醚、氯仿、乙酸乙酯、正丁醇部位的抑菌作用。结果表明,石油醚、氯仿、乙酸乙酯部位有抑菌作用,氯仿部位对金黄色葡萄球菌、绿脓杆菌、杀螟杆菌、大肠杆菌及枯草杆菌抑菌作用最强,最小抑菌浓度为0.026~0.103 mg/片;石油醚、乙酸乙酯部位最小抑菌浓度均为0.026~0.412 mg/片;挥发油对5种供试菌均有抑制作用,最小抑菌浓度为0.412~0.823 mg/片。Bacteriostasis test was conducted to study the antimicrobial activity of petroleum ether,chloroform,ethyl acetate,n-butanol extracts of the bark from Clausena anisum-olens by disk diffusion method.Results showed that the petroleum ether,chloroform and ethyl acetate extracts had antimicrobial activity,and the chloroform extracts had the strongest activity against Staphylococcus aureus,Pseudomonas aeruginosa,Bacillus cereus,Escherichia coli,B.subtilis with a minimal inhibitory concentration（MIC） of 0.026~0.103 mg/piece,and petroleum ether extracts and ethyl acetate showed stronger activity with the MIC of 0.026~0.412 mg/piece.The volatile oil had antimicrobial activity against the five tested bacilus with the MIC of 0.412~0.823 mg/piece.

关 键 词：细叶黄皮[Clausena anisum-olens(Blanco.)Merr]茎皮 提取物 最小抑菌浓度 

分 类 号：S482.292[农业科学—农药学]