益气活血法对脑出血大鼠脑内凝血酶敏感蛋白-1及其受体CD36表达的影响  

Effects of Replenishing Qi and Promoting Blood Circulation Treatment on the Expression of Thrombospondin-1 and Receptor CD36 of Intracerebral Hemorrhagic Rats

作　　者：陈柏林[1] 邢之华[1] 唐涛[1] 刘宜峰[1] 阳鹤鹏[1] 刘清娥[1] 吴汉军[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《南京中医药大学学报》2011年第4期342-345,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学青年基金(30400581);中国博士后科学基金(2005038224);湖南省博士后专项资助计划(2006FJ4244);湖南省青年骨干教师培养对象经费(湘教通[2005]247号)

摘　　要：目的通过观察益气活血法对脑出血大鼠脑内损伤区凝血酶敏感蛋白-1(thrombospondin-1,TSP-1)及其受体CD36表达的影响,初步探讨益气活血法治疗脑出血的机制。方法将155只SD大鼠随机分为正常组、假手术组、模型组、益气活血组、益气组和活血组;用Ⅶ型胶原酶诱导脑出血大鼠模型,再对相应的组分别灌服补阳还五汤及该方的益气药组和活血药组,在不同的时间点取脑组织并用免疫印迹(Western-blotting)分析鼠脑TSP-1及CD36的表达。结果正常组和假手术组不同时间点TSP-1和CD36表达无明显变化。模型组TSP-1在脑出血后4 d达高峰,CD36分别在脑出血后4、28 d达高峰。益气活血组1 d TSP-1表达低于模型组(P<0.01),4 d TSP-1和CD36表达均低于模型组(P<0.01),28 d CD36表达高于模型组(P<0.01)。结论益气活血法有可能通过影响脑出血后损伤区TSP-1及其受体CD36的表达,降低其对血管新生的抑制作用,促进新生血管成形、成熟和脑组织修复。OBJECTIVE To make a primary study of the mechanism of replenishing qi and promoting blood circulation treatment for curing intracerebral hemorrhage（ICH） rats by observing the expression of thrombospondin-1（TSP-1） and its receptor CD36.METHODS 155 SD rats were randomly divided into six groups,namely normal group,sham operated group,ICH model group,replenishing qi and promoting blood circulation treatment group,replenishing qi group and promoting blood circulation treatment group.ICH model was established by injecting collagenase type Ⅶ,while replenishing qi group,promoting blood circulation treatment group and replenishing qi and promoting blood circulation treatment group were respectively administered with replenishing qi decoction,promoting blood circulation treatment decoction and Buyang Huanwu Decoction.Afterwards,brain tissue was extracted different and the expression of TSP-1 and CD36 were detected by western blotting.RESULTS There was no significant changes in the expression of TSP-1 and CD36 between normal group and sham operated group at different time.In ICH model group,TSP-1 reached peak on the fourth day,CD36 on the 4th and 28th day.In replenishing qi and promoting blood circulation treatment group,the expression of TSP-1 was lower than that in model group on the 1st day（P0.01）;the expression of TSP-1 and CD36 was both lower than that in model group on the 4th day（P0.01）;the expression of CD36 was higher than that in model group on the 28th day（P0.01）.CONCLUSION Replenishing qi and promoting blood circulation treatment may regulate the expression of TSP-1 and its receptor CD36 in ICH rat,thus it can lower the inhibiting effect on angiogenesis and promote the shaping and maturity of new blood vessels as well as the recovery of brain tissue injury.

关 键 词：脑出血 益气活血法 凝血酶敏感蛋白-1 受体 免疫印迹 

分 类 号：R743.34[医药卫生—神经病学与精神病学]