芪丑汤对肾间质纤维化大鼠EMT抵抗作用研究  

Study on resistant effect of Qichoutang on renal tubular epithelial cell transdifferentiation in renal interstitial fibrosis in rats

作　　者：张翠[1] 张兰[1] 刘颖[1] 王晓泊[1] 王萌[1] 代百东[1] 

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2011年第3期267-271,319,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省教育厅基金项目(11531451)

摘　　要：研究芪丑汤对肾间质纤维化大鼠肾小管上皮细胞转分化的抵抗作用,探讨其抗肾间质纤维化的作用机制.采用单侧输尿管梗阻(UUO)诱导大鼠肾间质纤维化动物模型,以依那普利为阳性对照药,将大鼠随机分为假手术组,模型组,依那普利组,芪丑汤高剂量组,中剂量组,低剂量组.术后第12天处死大鼠,收集血清测定肌酐(Scr),尿素氮水平(BUN),总胆固醇(CHO),甘油三酯(TG);测定红细胞(RBC),血红蛋白(Hb);采用免疫组化半定量分析法检测各组大鼠肾间质α-平滑肌肌动蛋白(α-SMA),Ⅲ型胶原(ColⅢ)的表达.肾组织采用HE,VG染色评定肾小管间质损伤及胶原沉积程度.模型组α-SMA,ColⅢ表达,BUN、Scr,CHO,TG,RBC,Hb及肾小管间质损伤指数,胶原沉积程度高于假手术组(P<0.01),芪丑汤与依那普利组比较α-SMA,ColⅢ,BUN,Scr,CHO,TG,RBC,Hb的表达均明显降低(P<0.05),肾小管间质损伤指数及胶原纤维沉积减轻.芪丑汤通过下调α-SMA,ColⅢ,BUN,Scr,CHO,TG的表达,升高RBC,Hb,降低肾小管间质损伤指数及胶原沉积,阻止肾小管上皮细胞向间充质细胞的转分化作用,从而减缓肾间质纤维化进程.To investigate the possible preventive mechanism of Qichoutang on renal tubular epithelial cell transdifferentiation in renal interstitial fibrosis in rats. Using unilateral ureteral obstruction UUO induction renal interstitial fibrosis animal models with enalaprilat as the positive control medicine, rats were randomly divided into Sham, UUO, ACEI, Qiehoutang of high-dosage, mid-dosage and low-dosage groups. Rats were killed in 12th day after surgery, Ser, BUN, CHO, TG, RBC and Hb were collected from each group. The expression of the renal interstitial α- smooth muscle actin α- SMA and Ⅲ collagen Col Ⅲ of rats from each group were detected by semi -quantitative immunohistochemieal analysis. Measured the tubulointerstitial damage index and collagen deposition level of kidney tissues with HE, VG staining. In UUO rats, the tubular interstitial damage index, collagen deposition level, the expressions of α- SMA, eollagen Ⅲ （Col Ⅲ）, BUN, Scr, CHO, TG, RBC, Hb were all increased compared with thse of Sham group（P 〈0.01 ）. Qiehoutang significantly decreases the expressions of α-SMA, ColⅢ, BUN, Ser, CHO, TG, RBC, Hb compared with ACEI rats （P 〈 0.05 ） and ameliorates tubulo interstitial damage index, collagen deposition. In renal interstitial fibrosis - induced UUO rats, Qichoutang may retard the progression of renal fibrosis by down regulation the expression of α- SMA, LN, ColⅢ, BUN, Scr, CHO, TG, decrease tubulointerstitial damage index and collagen deposition and prevent transdifferentiation action from renal tubular epithelial cells to mesenchyme cell.

关 键 词：芪丑汤 肾间质纤维化 大鼠 肾小管上皮细胞转分化 

分 类 号：R285.5[医药卫生—中药学]