兔蛛网膜下腔出血早期脑损伤模型的建立及NO作用的初步研究  

Early brain injury model of spontaneous subarachnoid hemorrhage in rabbit and preliminary study on the role of NO

作　　者：许晖[1] 王业忠[1] 范高阳[1] 刘祺[1] 姬云翔[1] 田卫东[1] 姚俊岭[1] 赵冬[1] 

机构地区：[1]新疆石河子大学医学院第一附属医院神经外科,832008

出　　处：《临床神经外科杂志》2011年第3期116-118,共3页Journal of Clinical Neurosurgery

摘　　要：目的建立一种可重复的、与临床相吻合的症状性蛛网膜下腔出血(SAH)早期脑损伤(EBI)的动物模型,初步探讨NO在EBI中的作用。方法选用新西兰大耳白兔60只,随机分为实验组、对照组、空白组。采用枕大池穿刺一次注入自体动脉血法建立SAH模型。结果实验组枕大池和蛛网膜下腔内发现大量的血凝块和血液。实验组光镜下可观察到蛛网膜下腔大量红细胞,神经元细胞水肿,电镜下可见胶质细胞空泡样改变,神经元细胞粗面内质网扩张,线粒体肿胀,溶酶体增多。实验组血浆NO浓度于造模后12 h与对照组和空白组比较均有显著差异性(P<0.01);造模后4 h、24 h、48 h、72 h:实验组与对照组和空白组比较无差异性。对照组和空白组造模后无时相性变化。结论枕大池一次注血法是一可靠的自发性SAH模型,适宜于研究SAH后早期脑损伤,NO在EBI中发挥一定的作用。Objective To build a repeatable and anastomosisable animal model of symptomatic subarachnoid hemorrhage （SAH） in rabbit. Methods 60 rabbits were randomly divided into three groups including experimental group, control group and blank group. Autologous arterial blood was injected into cisternal puncture one time. Results Blood clots and blood were found in subarachnoid and cisterna magna, appearance significant neurological symptoms in the postoperative. Subarachnoid space could be observed under the light microscope a lot of red blood cells, nerve cells edema; In the electron microscope, vacuolar changes of glial cells, nerve cells expanded rough endoplasmic reticulum, mitoehondria swelling, increased lysosomes, myelin memory in the vacuole and lamellar separation. NO concentration in the plasma of experimental group after modeling 12h in the control group and blank group were significantly different （ P 〈 0.01 ） ; after the model 4h, 24h, 48h, 72h： experimental group and control group and blank group There was no difference. The control group and the blank group after the modeling phase of no change. Conclusion The method of cisternal puncture injection blood one time is a simple, reliable and repeatable model of spontaneous SAH, suitable for research EBI, NO in the EBI play a role.

关 键 词：自发性蛛网膜下腔出血 早期脑损伤 动物模型 NO 

分 类 号：R743.35[医药卫生—神经病学与精神病学]