阴离子色谱-积分脉冲安培法测定珍稀药材金线莲中游离氨基酸  

Determination of Free Amino Acids in Precious Substances of Anoectochilus Roxburghii by Anion Chromatography-Integral Pulse Ampere

作　　者：钟添华[1] 黄丽英[2] 房静 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]福建医科大学,福建福州350004 [3]天津市生物工程职业技术学院,天津300462

出　　处：《分析测试技术与仪器》2011年第2期74-78,共5页Analysis and Testing Technology and Instruments

基　　金：国家海洋局青年基金项目(编号:海三科2010135)

摘　　要：目的：定性及定量分析珍稀药材金线莲中的游离氨基酸.方法：将高效阴离子交换色谱-积分脉冲安培检测法应用于珍稀药材金线莲游离氨基酸的分析测定.结果：17种氨基酸的检出限在0.23~3.37 pmol之间,峰面积校正曲线的线性范围为2~3个数量级,5次平行测定的峰面积的相对标准偏差RSD在0.96%~4.2%之间.结论：方法适用于药材金线莲中氨基酸含量的测定,结果良好.干燥金线莲药材的加入回收率分别在88.9%~108.0%之间.Objective：The qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Method： The anion chromatography-integral pulse ampere method is used in the qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Results： The limits of detection（IOD） for 17 amino acids fell between 0.23~3.37 pmol,the linearity range of peak area calibration curve（profile） was the order of 2-3 and RSDs for five determinations were 0.96%~4.2%.Conclusion： This method is applicable to the determination of free amino acids in the medicinal material Anoectochilus roxburghii with good results.And the recovery of dried Anoectochilus roxburghii was 88.9%~108.0%.

关 键 词：阴离子色谱-积分脉冲安培法 金线莲 游离氨基酸 

分 类 号：O657.33[理学—分析化学]