白细胞介素6对前列腺癌细胞体外生长的影响及其调节  

The Effect of IL 6 on the growth of prostate cancer cells in vitro and its modulation

作　　者：邢毅飞[1] 肖亚军[1] 张齐钧[1] 鲁功成[1] 

机构地区：[1]同济医科大学附属协和医院泌尿外科,武汉430022

出　　处：《中华实验外科杂志》1999年第6期502-503,共2页Chinese Journal of Experimental Surgery

基　　金：卫生部默沙东基金

摘　　要：目的　了解白细胞介素６（ＩＬ６） 对前列腺癌（ｐｒｏｓｔａｔｅ ｃａｎｃｅｒ，ＰＣａ） 细胞体外生长的影响及地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ，Ｄｅｘ） 对此的调节。方法　采用ＥＬＩＳＡ 法测定ＰＣａ 细胞系ＰＣ３ｍ 细胞ＩＬ６ 的自分泌及Ｄｅｘ 处理后的分泌状况，并用结晶紫法观察ＩＬ６ 、抗ＩＬ６ 抗体和Ｄｅｘ 对ＰＣ３ｍ 细胞生长的影响。结果　ＰＣ３ｍ 细胞可自分泌ＩＬ６，ＩＬ６ 无促进ＰＣ３ｍ 细胞增殖作用（ Ｐ＞ ０．０５）；抗ＩＬ６ 抗体可抑制ＰＣ３ｍ 细胞生长，细胞毒性由（１４．８４ ±１．２５） ％ 递增至（４１ ．４１ ±０ ．８０）％ ，呈剂量依赖关系（ Ｐ＜０．０５） 。Ｄｅｘ 不能减少ＩＬ６ 的产生但可抑制ＰＣ３ｍ 细胞增殖。细胞毒性由（１４ ．０６ ±０．２０）％ 递增至（３１ ．２５ ±１ ．１９）％ ，呈浓度依赖关系（ Ｐ＜ ０ ．０５）。结论　ＩＬ６ 为ＰＣ３ｍ 细胞的自分泌生长因子；Ｄｅｘ 可抑制ＰＣ３ｍ 细胞增殖，但并非通过抑制ＩＬ６ 的生成而发挥作用。Objective\ To study the role of interleukin 6 (IL 6) in the pathogenesis of an androgen independent prostate cancer line (PC 3m) and the modulatory effect of dexamethasone. Methods\ The secretion of IL 6 by PC 3m cells treated without or with dexamethasone were measured by using a pretty sensitive and specific enzyme immunoassay (ELISA), and the effects of exogenous IL 6, anti IL 6 monoclonal antibody and dexamethasone on the proliferation of PC 3m cells were determined by applying crystal violet assay. Results\ PC 3m cells produced certain IL 6. Exogenous IL 6 had no obvious promotion on the proliferation of PC 3m cells ( P >0.05), while anti IL 6 monoclonal antibody could dose dependently inhibited the growth of PC 3m cells, with the cytotoxicity being increased from (14.84±1.25) % to (41.41±0.80) % ( P <0.05). Dexamethasone could not inhibit the production of IL 6, but dose dependently the proliferation of PC 3cm cells ( P <0.05) with the cytotoxicity being increased from (14.06±0.20) % to (31.25±1.19) %. Conclusion\ IL 6 acts as an autocrine growth factor for PC 3m, and dexamethasone can inhibit the growth of PC 3m cells by a mechanism independent of its effect on IL 6 mRNA expression.\;

关 键 词：白细胞介素6 前列腺肿瘤 地塞米松 

分 类 号：R737.25[医药卫生—肿瘤]