Rxa对L02细胞增殖的细胞周期分析和对Ki-67、Cyclin D1、PCNA蛋白表达的影响  

Effects of Rxa on L02 cell proliferation and the expression of Ki 67, Cyclin D1, PCNA protein

作　　者：卢绮萍[1] 吴在德[2] 王伟[1] 田磊[2] 李德忠[1] 

机构地区：[1]广州军区武汉总医院,武汉430070 [2]同济医科大学附属同济医院

出　　处：《中华实验外科杂志》1999年第6期512-514,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资;湖北省重点科技项目

摘　　要：目的　探讨丹参提取物Ｒｘａ 对正常肝细胞（Ｌ０２ 细胞） 增殖速率的影响。方法　应用流式细胞术（ＦＣＭ）分别测定Ｌ０２ 细胞Ｒｘａ 处理组（Ｌａ 组） 和未处理组（Ｌ组）０、２、４、８、１２ 小时Ｇ０Ｇ１ 、Ｓ、Ｇ２Ｍ 期细胞指数，同步进行Ｋｉ６７、Ｃｙｃｌｉｎ Ｄ１ 、ＰＣＮＡ 蛋白表达的免疫组化分析。结果　Ｌａ 组细胞增殖速率较快，Ｋｉ６７ 抗原表达相对较强，ＣｙｃｌｉｎＤ１、ＰＣＮＡ 表达相对提前。结论　Ｒｘａ 可能促进Ｌ０２ 细胞增殖。Objective\ To investigate the effect of Rxa, a kind of extract of RSM, on the proliferation rate in cultured human hepatocytes (L02 cells). Methods\ L02 cells were divided into two groups: non treatment group (L group) and Rxa treated group (La group, Rxa 2 mmol/L). Indices of G0 G1 phase cells, S phase cells and G2 M phase cells were measured by using flow cytometry at 0, 2, 4, 8 and 12 h, respectively, and the expression of Ki 67, Cyclin D1, PCNA protein were simultaneously detected by using immunohistochemical method. Results\ Index of hepatocytes in each phase reached the peak more quickly in Rxa treated group than in non treatment group, and the Ki 67 expression was also higher than that one. Cyclin D1 and PCNA expressed earlier in the hepatocytes treated with Rxa ( P <0.01), which expressed sooner than in the hepatocytes not treated with Rxa about 2～4 h. Conclusion\ Rxa may be play an important role in the hepatic proliferation, which may be contributed to the triggering expression of Ki 67, Cyclin D1 and PCNA, by which the cell cycle transport was speeded up.\;

关 键 词：肝细胞 细胞增殖 细胞周期 丹参提取物 PCNA 

分 类 号：R575[医药卫生—消化系统] R285[医药卫生—内科学]