法国海岸松树皮提取物碧萝芷对长链游离脂肪酸诱导的巨噬细胞perilipin2基因表达的影响  

Pycnogenol,an Extract from French Martime Pine,Suppresses Oleic Acid-induced perilipin2 Expression in Macrophages

作　　者：范斌[1] 杜强[2] 谷剑秋[3] 张锦[3] 

机构地区：[1]中国医科大学附属盛京医院神经内科,沈阳110004 [2]中国医科大学附属盛京医院内分泌科,沈阳110004 [3]中国医科大学附属第一医院内分泌科,沈阳110001

出　　处：《中国医科大学学报》2011年第7期611-615,共5页Journal of China Medical University

摘　　要：目的研究碧萝芷(PYC)对油酸诱导的巨噬细胞perilipin2表达的影响及其相关分子机制。方法应用Real-time PCR和Western blot测定油酸及PYC对perilipin2mRNA和蛋白水平表达影响。应用荧光素酶活性分析方法检测油酸及PYC对perilipin2启动子活性的影响。结果油酸以剂量和浓度依赖方式上调perilipin2mRNA和蛋白水平表达,并促进perilipin2启动子活性。PYC以剂量依赖方式抑制了油酸诱导的perilipin2表达及启动子活性。结论 PYC抑制了巨噬细胞中油酸诱导的perilipin2的表达。PYC通过抑制perilipin2启动子活性,从而直接抑制perilipin2的表达。Objective To investigate the effect of Pycnogenol on oleic acid-induced perilipin2 expression in macrophages.Methods Real-time PCR and Western blot were performed to detect perilipin2 expression.Transient transfection and luciferase assay were employed to measure perilipin2 promoter activity.Results Oleic acid significantly induced perilipin2 expression in a dose-and time-dependent manner in macrophages;oleic acid markedly enhanced perilipin2 promoter activity;Pycnogenol significantly suppressed oleic acid-induced perilipin2 expression and promoter activity.Conclusion For the first time,we demonstrated that Pycnogenol significantly suppressed oleic acid-induced perilipin2 expression and promoter activity.

关 键 词：长链游离脂肪酸 泡沫细胞 碧萝芷 PAT家族蛋白 

分 类 号：R363[医药卫生—病理学]