重组腺病毒r-caspase3的靶向性实验研究  

The Study of The Effection of Hepatocellular Carcinoma-Targeting Adenovirus On The AFP Expression of Hepatocellular Carcinoma Cells

作　　者：王炜[1] 沈浩元[1] 刘猛[1] 

机构地区：[1]广州医学院第一附属医院肝胆外科,510120

出　　处：《实用医学杂志》2011年第14期2506-2508,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:30571829);广东省医学科研基金(编号:A2006282)

摘　　要：目的:观察构建靶向性反向半胱氨酸蛋白酶3(r-caspase3)重组腺病毒对AFP表达阳性肝癌细胞凋亡诱导的效果和特异性。方法:采用AFP表达强阳性细胞HepG2、表达弱阳性细胞7721、正常肝细胞L-02以及AFP表达阴性乳腺癌细胞MDA-MB-231,分别感染构建之重组腺病毒Ad-r-caspase3、Ad-EAFPPALB/r-caspase3及Ad-PALB/r-caspase3,SRB染色测定O.D值,计算细胞死亡率和IC50;流式细胞术检测细胞凋亡指数。结果:HepG2、7721细胞对各腺病毒敏感程度为Ad-EAFPPALB/r-caspase3>Ad-PALB/r-caspase3>Ad-r-caspase3;流式细胞术检测结果显示重组腺病毒Ad-EAFPPALB/r-caspase3具有极强的凋亡选择性,AFP表达越强,凋亡指数越高。结论:靶向性Ad-EAFP-PALB/r-caspase3重组腺病毒具有肝细胞性肝癌凋亡选择性,同AFP表达呈正相关。Objective To observed the therapic apoptosis efl^etion of Hepatoeellular Carcinoma-Targeting Adenovirus Containing r-caspase3 gene which on AFP expressed hepatocellular carcinoma. Methods The AFP expressed cells HepG2,7721,normal hepatocyte L-02 and breast cancer cells MDA-MB-231 were lot）coted by recombinant Adenovirus Ad-r-caspase3,Ad-EAFP-PPAB/r-caspase3 and Ad-PAB/r-caspase3, the O.D figure were detected by SRB dyeing, and the apoptotic figure were tested by FCM. Results The sensitivity alter infection with Adenovirus on HepG2 and 7721 showed： Ad-EAFPPALB,/r-caspase3 〉 Ad-PALB/r-easpase3 〉 Ad-r-easpase3;the data of FCM showed：the recombinant Adenovirus of Ad-EAFP.PAFB/r-caspase3 has very strong apoptotic specificity on AFP expressed cell. Conclusion The Recombinant of Hepatoeellular carcinoma-targeting Adenovirus Containing r-caspase3 gene has apoptotic specificity on AFP expressed cell and the more stronger expression the more apoptotie specificity.

关 键 词：癌 肝细胞 腺病毒 反向半胱氨酸蛋白酶3基因 靶向性 

分 类 号：R735.7[医药卫生—肿瘤]