AnnexinⅠ表达下调与鼻咽癌分化转移的关系  

Annexin Ⅰ Down-Regulation in Nasopharyngeal Carcinoma is Associated with Epithelial Differentiation and Metastasis

作　　者：程爱兰[1] 彭娟[1] 张杨[1] 唐海林[1] 唐运莲[1] 

机构地区：[1]南华大学肿瘤研究所肿瘤细胞与分子病理学重点实验室,湖南衡阳421001

出　　处：《中南医学科学杂志》2011年第3期253-257,共5页Medical Science Journal of Central South China

基　　金：国家自然科学基金(81072198);湖南省教育厅重点项目(10A104);湖南省自然科学基金(10JJ6035)

摘　　要：目的检测膜联蛋白Ⅰ(Annexin Ⅰ)在正常鼻咽黏膜上皮组织(NNET)、原发鼻咽癌(NPC)组织及颈淋巴结转移鼻咽癌(LMNPC)组织中的表达,探讨其临床病理学意义。方法采用免疫组织化学S-P法检测30例NNET、98例原发NPC组织(高分化鳞癌5例,中度分化鳞癌13例,低分化和未分化癌80例)及20例LMNPC组织中Annexin Ⅰ蛋白的表达情况;采用SPSS13.0软件包对数据进行非参数检验。结果与NNET比较,Annexin Ⅰ在原发NPC组织中的表达明显下调(P<0.05);与原发NPC比较,Annexin Ⅰ在LMNPC中的表达水平明显下调(P<0.01);98例原发NPC组织标本中,Annexin Ⅰ的表达水平与NPC的病理分化程度、临床分期、复发及局部淋巴结和远处转移有关,Annexin Ⅰ低表达的肿瘤较高表达的肿瘤分化差,有更晚的临床分期、更容易复发、更容易发生局部淋巴结和远处转移(P<0.05 or 0.01)。结论Annexin Ⅰ与NPC的分化程度、转移和预后相关,有望成为预测鼻咽癌转移、预后和区别NPC分化程度的分子标志物。提示Annexin Ⅰ在鉴别NPC组织学分化程度、预测鼻咽癌病人预后及预警鼻咽癌转移和复发方面有一定的临床价值。Objective To investigate the expression of annexin I in normal nasopharyngal epithelial tissue（ NNET）, human primary nasopharyngeal carcinoma（NPC） and cervical lymph node metastases NPC （LMNPC）, and to determine its relationship with clinic pathological characters. Methods Immunohistochemistry was performed to detect the expression of annexin I in 30 cases of normal nasopharyngal epithelial tissue,98 cases of human primary NPC tissue specimens, and 20 cases of cervical lymph node metastases NPC, and the correlation of its expression level with clinicopathologic features and clinical outcomes were evaluated with SPSS13.0 software package for non-parametric analysis. Results Annexin I was significantly down-regulated in NPC versus normal nasopharyngalepithelial tissue（ P 〈 0.05 ）. The expression level of annexin I in primary NPC was significantly higher than those in lymph node metastases NPC（P 〈 0.01 ）. Annexin I down-regulation was correlated with poor histologic type/grade, advanced clinical stage, recurrence, regional lymphonode and distant metastasis（ P 〈 0.01 ）, tumors with annexin I down-regulation tended to have a poor histological differentiation, more advanced clinical stage, more frequent recurrences, more liability lymph node and distant metastasis （ P 〈 0.05 or 0.01 ）. Conclusion The results suggest that annexin I is the potential biomarker for differentiation, metastasis, and prognosis of NPC, and might contribute to NPC carcinogenesis. These findings reported here could have clinical value in distinguishing histological grades, predicting the prognosis of NPC and identifying NPC patients that are at high risk of progression and recurrence.

关 键 词：ANNEXINI 鼻咽癌 分化 转移 

分 类 号：R739.6[医药卫生—肿瘤]