兔VX2肌肿瘤模型制作方法的改良及超声评价  

Improvement of VX2 muscle tumor rabbit models establishment and ultrasonic evaluation

作　　者：吴菲[1] 曹兵生[2] 邓娟[2] 史迎昌[3] 

机构地区：[1]河北北方学院研究生部,河北张家口075000 [2]中国人民解放军第309医院超声科,北京100091 [3]中国人民解放军第309医院实验动物中心,北京100091

出　　处：《中国医学影像技术》2011年第7期1340-1343,共4页Chinese Journal of Medical Imaging Technology

摘　　要：目的改良VX2肌肿瘤模型的制作方法,并采用超声对肿瘤进行评价。方法健康新西兰兔20只,随机分为超声引导组和切口包埋组,每组10只,于实验兔双侧后肢肌肉行VX2肿瘤种植,超声引导组采用超声引导下组织块接种法,切口包埋组采用切口组织块包埋法,每侧种植约1 mm3的VX2瘤块。观察比较两组肿瘤接种所需时间、成瘤率,且分别于种植后第7、14、21、26天进行超声检查,观察肿瘤生长情况。结果超声引导组及切口包埋组肿瘤接种所需时间分别为(93.78±7.42)s与(251.81±17.87)s,成瘤率分别为100%(20/20)、80.00%(16/20),两组间差异有统计学意义(P<0.05);超声引导组与切口包埋组接种成功的肿瘤均为单发,且大小均一。结论超声引导下接种1 mm3大小瘤块建立兔VX2肌肿瘤模型操作简单、快速、成功率高、肿瘤生长均一,是实验研究肿瘤介入治疗的理想动物模型。Objective To improve the establishment of rabbit model with VX2 muscle tumor,and to evaluate the tumor with ultrasonography.Methods Twenty New Zealand white rabbits were randomly divided into two groups（each n=10）.Both sides of rabbit thigh muscles were inoculated under the guidance of ultrasound（ultrasound-guidance group） or via incision route（incision group）,respectively.For every side,the active VX2 tumor tissue to inoculate was cut to about 1 mm3.The tumor growth rate and the inoculation time were observed.Ultrasonography was performed at the 7th,14th,21st and 26th day after inoculation to monitor the tumor growth.Results The time of inoculation was（93.78±7.42）s and（251.81±17.87）s,respectively（P0.05）.The tumor growth rate of ultrasound-guidance group and incision route group was 100%（20/20） and 80.00%（16/20）,respectively（P0.05）.All tumors of two groups were solitary and uniformed.Conclusion The method of ultrasound-guided implantation with one block of 1 mm3 active tumor tissues for establishing rabbit VX2 muscle tumor model is simple,time-saving with high success rate and growth-uniformed,therefore,it is valuable to interventional therapy experiments.

关 键 词：肌肉肿瘤 模型 动物 超声检查 

分 类 号：R738.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]