缓激肽对大鼠脑胶质瘤微血管中NF-κ B活性和表达的影响  

Effect of bradykinin on activity and expression of NF-κ B in microvessels of brain glioma of rat

作　　者：刘丽波[1] 马腾[1] 陈方圆[2] 解辉[1] 薛一雪[1] 

机构地区：[1]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001 [2]中国医科大学,92期七年制辽宁沈阳110001

出　　处：《解剖科学进展》2011年第4期389-392,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(30973079;81072056;30872656;30670723);沈阳科学技术计划资助项目(F10-205-1-37;F10-205-1-22);教育部博士学科点专项科研基金(20092104110015;20102104120023)

摘　　要：目的研究缓激肽(bradykinin,BK)对脑胶质瘤大鼠肿瘤微血管内皮细胞中转录因子核因子-κ B(nuclearfactor-kappaB,NF-κ B)的活性和表达的影响。方法雌性Wistar大鼠96只,随机分为6组,即对照组、BK 5min组、BK 10min组、BK 15min组、BK 30min组和BK 60min组。每组16只。建立大鼠C6胶质瘤模型,颈动脉给药,应用Trans AMTMNF-κ B p65试剂盒检测NF-κ B(p65)的活性;应用免疫组织化学法检测大鼠脑肿瘤微血管上NF-κ B(p65)的分布和表达水平变化;应用western blot法检测肿瘤微血管内皮细胞中NF-κ B(p65)蛋白表达水平的变化。结果与对照组相比,BK显著增强了NF-κ B的活性;在脑肿瘤组织,NF-κ B(p65)主要表达在肿瘤微血管和肿瘤细胞上,BK能够增加NF-κ B(p65)在肿瘤微血管上的表达,并且上调肿瘤微血管内皮细胞细胞核中NF-κ B(p65)的蛋白表达,在BK作用15min时效果最显著(P<0.01)。结论缓激肽能够增强脑胶质瘤大鼠肿瘤微血管中NF-κ B的活性和表达水平,NF-κ B可能是BK开放血肿瘤屏障机制之一。Objective To investigate the effect of bradykinin （BK） on the activity and expression of nuclear factor-kappa B （NF-кB） in microvascular endothelial cells of brain glioma rat.Methods 96 female rats were divided averagely into 6 groups：control,BK 5min group,BK 10min group,BK 15min group,BK 30min and BK 60min groupd.The C6 brain glioma rat model was established and BK was pumped into rats,brain via the carotid artery,trans AMTM NF-κB p65 Kit was used to measure the activity of NF-kB.The distribution and expression of NF-кB （p65） in tumor microvessels were detected by SP immunohistochemistry and Western blot respectively.Results In contrast to control group,BK obviously enhanced the activity of NF-кB （p65）.In glioma rats,model,NF-кB （p65） mainly expressed in tumor microvessels and tumor cells,and the expression level of NF-кB （p65） in tumor microvessels was increased after BK infusion compared with glioma rats,model.Moreover,BK could upregulate the expression of NF-кB （p65） protein in the nucleus of tumor microvascular endothelial cells and reach peak at BK infusing for 15min （P〈0.01）.Conclusions BK could increase the activity and expression of NF-кB （p65） in microvessels of brain glioma rats,NF-кB might be involved in the mechanism of BK opeining the brain tumor barrier.

关 键 词：缓激肽 NF-ΚB 血肿瘤屏障 大鼠 

分 类 号：R-338.2[医药卫生] R341.6