嗅鞘细胞移植修复坐骨神经缺损  

Olfactory ensheathing cell transplantation for treatment of sciatic nerve injury

作　　者：杨硕[1] 侯占江[1] 夏昊晨[1] 樊昌东[1] 

机构地区：[1]哈尔滨医科大学附属第一医院急诊创伤外科,黑龙江省哈尔滨市150001

出　　处：《中国组织工程研究与临床康复》2011年第19期3503-3506,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:研究表明,嗅鞘细胞有利于神经元存活并促进轴突再生。目的:验证局部注射嗅鞘细胞治疗大鼠周围神经损伤的可行性。方法:体外分离、培养SD大鼠嗅鞘细胞。40只SD大鼠切除坐骨神经1.0cm,植入异体神经1.0cm。随机分为2组,嗅鞘细胞组局部注射嗅鞘细胞,生理盐水组局部注射生理盐水。术后3个月检测体感诱发电位及运动诱发电位,光镜、电镜观察神经电生理恢复情况。结果与结论:电镜观察嗅鞘细胞组大鼠在损伤区有较多神经纤维通过,明显多于生理盐水组(P<0.01)。嗅鞘细胞组大鼠体感诱发电位及运动诱发电位的潜伏期及波幅明显优于生理盐水组(P<0.01)。提示局部注射嗅鞘细胞能更好地恢复周围神经损伤后的功能。BACKGROUND： Studies have demonstrated that olfactory ensheathing cells （OECs） are conducive to the survival of neurons and promote axonal regeneration.OBJECTIVE： To study the feasibility and effect of local injection of rat OECs to treat peripheral nerve injury.METHODS： Rat OECs were isolated and cultured in vitro.1.0 cm sciatic nerve segment was cut from 40 adult female SD rats undergoing allograft nerve transplantation,and then the rats were randomly divided into two groups.OECs group： local injection of OECs;saline group： local injection of sterile saline.After 3 months,somatosensory evoked potentials （SEP） and motor evoked potentials （MEP） were detected and electrophysiological recovery of nerve was observed under light microscopy and electron microscopy.RESULTS AND CONCLUSION： The electron microscopy found that in the damage zone,OECs group had more nerve fibers significantly than saline group （P 0.01）;In OECs group,SEP and MEP latency and amplitude was significantly better than that in saline group （P 0.01）.it is shown that local injection of OECs can better restore function after peripheral nerve injury.

关 键 词：周围神经损伤 嗅鞘细胞 移植 体感诱发电位 运动诱发电位 

分 类 号：R394.2[医药卫生—医学遗传学]