Telocytes在大鼠心脏不同部位的分布  

Distribution of Telocytes in the rat heart

作　　者：刘娟娟[1,2,3] 沈晓涛[1,2,3,4] 郑馨[1,2,3] 李震[1,2,3] 王健[1,2,3] 齐绪峰[1,2,3] 蔡冬青[1,2,3] 

机构地区：[1]暨南大学再生医学教育部重点实验室,广东省广州市510632 [2]暨南大学再生医学香港中文大学暨南大学联合实验室,广东省广州市510632 [3]暨南大学科技部及广东省科技厅国际科技合作基地,广东省广州市510632 [4]暨南大学生物医学工程系,广东省广州市510632

出　　处：《中国组织工程研究与临床康复》2011年第19期3546-3548,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：课题受863项目(2007AA02Z105);国家自然科学基金(30770886,30570369,30340038,30973158);广东省自然科学基金重点项目(04105826);广东省科技攻关项目(2004B30601007);广东省国际合作项目(2009B050900007);中央高校基本科研业务费专项基金(21609408)的资助~~

摘　　要：背景:心脏Telocytes可能是心肌细胞及心脏内源性干细胞的联系及支持细胞,在心肌损伤后的修复与再生和心室重构中起重要作用,但目前对Telocytes在心脏中的分布与相应的功能所知甚少。目的:观察Telocytes在大鼠心脏不同部位的分布情况。方法:取成年SD雌性大鼠心脏,行横断面连续冰冻切片,分别取心脏心底部、心脏中间部、心房-心耳部分的代表性切面进行抗c-kit免疫荧光染色,观察Telocytes在心脏的分布情况。结果与结论:结果发现心底部(26个/mm2)和心房-心耳部(20个/mm2)的Telocytes密度明显多于中间部(9个/mm2)。上述三个代表性横断面,其心肌外层区域Telocytes密度(心底部:18个/mm2;中间部:5个/mm2;心房-心耳部:13个/mm2)显著多于心肌内层区域(心底部:7个/mm2;中间部:4个/mm2;心房-心耳部:7个/mm2)。结果提示心脏Telocytes在心脏不同部分的分布不同,可能与心肌细胞及其内源性干细胞在上述区域分布不同有关。BACKGROUND： Telocytes in the heart are possible to act as linker and supporting cells between cardiac myocytes and endogenous cardiac stem cells,and play an important role in myocardial regeneration and ventricular remodeling after myocardial infarction （MI）.However,the distribution of Telocytes in the heart and their functional role are still unclear.OBJECTIVE： To investigate the distribution of Telocytes in the rat heart.METHODS： The continuous frozen sections were conducted using female SD rat heart.The representation sections from the base,the medium,and the atrium-atria of the heart were selected for immunofluorescence staining with c-kit antibody.The c-kit positive and unique morphology of Telocytes were applied as standard to identify the distribution of Telocytes in the rat heart.RESULTS AND CONCLUSION： The cell density of Telocytes in the base part （26 cells/mm2） and the atrium-atria part （20 cells/mm2） was significantly higher than that in the medium part （9 cells/mm2）.In addition,the cell density of Telocytes in the subepicardium （the base： 18 cells/mm2;the medium： 5 cells/mm2;the atrium-atria： 13 cells/mm2） was significantly higher than that in the endocardium （the base： 7 cells/mm2;the medium： 4 cells/mm2;the atrium-atria part： 7 cells/mm2）.The above-mentioned results suggested that the distribution of Telocytes in the heart is different.The pattern of Telocytes distribution in the heart may be related to the different distribution of cardiac myocytes and endogenous cardiac stem cells.

关 键 词：心肌内源性干细胞 TELOCYTES 心肌 分布 解剖 

分 类 号：R394.2[医药卫生—医学遗传学]