第一鳃弓外胚间充质细胞定向脂肪细胞的分化  

Directed differentiation of ectomesenchymal cells isolated from the first branchial arch into adipocytes

作　　者：侯景秋[1,2] 闫征斌[1,2] 杨旭[2] 

机构地区：[1]大庆油田总医院口腔科,黑龙江省大庆市163001 [2]佳木斯大学口腔医学院,黑龙江省佳木斯市154004

出　　处：《中国组织工程研究与临床康复》2011年第19期3559-3562,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:第一鳃弓作为过渡结构,在牙颌面的发育形成过程中,发挥着重要作用。目的:观察第一鳃弓外胚间充质细胞自我更新能力,以及体外诱导分化成为脂肪细胞的可能性。方法:分离培养E9.5Balb/c胎鼠第一鳃弓外胚间充质细胞,以端粒酶原位杂交和BrdU渗入实验检测外胚间充质细胞的增殖能力。相差显微镜下观察细胞形态变化和生长规律,免疫细胞化学染色鉴定细胞表面标志,cDNA探针原位杂交、免疫荧光化学染色观察细胞增殖能力,油红O染色、RT-PCR产物凝胶电泳观察细胞成脂诱导结果。结果与结论:端粒酶原位杂交和BrdU摄取实验阳性,诱导后细胞表现出典型的脂肪细胞形态,大部分细胞油红O染色阳性,RT-PCR结果显示诱导后细胞表达了脂蛋白脂酶。提示第一鳃弓外胚间充质细胞具有较高的自我更新能力,并可定向诱导分化成为脂肪细胞。BACKGROUND： During the dentofacial development,the first branchial arch,as a transitional structure,plays an important role.OBJECTIVE： To investigate the self-renewal of ectomesenchymal cells （EMCs） isolated from the first branchial arch as well as the feasibility of inducing EMCs into adipocytes.METHODS： The activity of proliferation and self-renewal of EMCs were detected by nuclei labeled with BrdU and fluorescence in situ hybridization for telomerase.Monolayer cultures of EMCs were passaged 3 times and then transferred to adipogenic media.The level of differentiation was evaluated by histological examination and the expression of tissue-specific genes was analyzed by reverse transcription-polymerase chain reaction technique.RESULTS AND CONCLUSION： Nuclei labeled with BrdU and fluorescence in situ hybridization for telomerase were positive.After being cultured in adipogenesis-inducing medium,EMCs responded by the accumulation of lipid vacuoles and the gene expression of LPL.The findings indicate that EMCs have a high activity of proliferation and self-renewal,and they are capable of differentiation into adipocytes in vitro.

关 键 词：第一鳃弓 间充质细胞 诱导 分化 脂肪细胞 

分 类 号：R394.2[医药卫生—医学遗传学]