淫羊藿素体外抗淋巴瘤细胞增殖效应  

Effect and Mechanism of Icaritin(ICT) on EL-4 Cells in Vitro

作　　者：范双翼[1] 余英豪[1] 

机构地区：[1]南京军区福州总医院病理科,福州350025

出　　处：《中国比较医学杂志》2011年第6期6-11,87,共7页Chinese Journal of Comparative Medicine

基　　金：南京军区医学科技研究"十一五"计划课题资助(07Z034);福建省自然科学基金(2010J01221)

摘　　要：目的本实验以小鼠T细胞淋巴瘤细胞株EL-4细胞株为模型,研究淫羊藿素(icaritin,ICT)是否抑制瘤细胞增殖,并初步探讨其作用机制。方法应用MTT比色法、透射电镜技术及流式细胞术检测ICT对EL-4细胞增殖能力的影响;用RT-PCR技术、比色法分析ICT的作用机制。结果 ICT对EL-4细胞增殖具有明显抑制作用,并呈量效及时效关系。透射电镜及流式细胞术结果显示,ICT可诱导EL-4细胞凋亡。RT-PCR显示,ICT作用于EL-4细胞后,bcl-2、P21基因的mRNA表达下调,Caspase-3、Caspase-9酶活性显著增强。结论 ICT可抑制体外培养的小鼠T淋巴瘤EL-4细胞的增殖并诱导其凋亡,可能系通过下调bcl-2、P21 mRNA表达,激活Caspase-3、Caspase-9蛋白等途径实现的。Objective To investigate the effect and probable mechanism of icaritin（ICT） on EL-4 cells in vitro.Methods MTT、transmission electron microscope（TEM）、flow cytometry analysis were used to examine the effect of ICT on the proliferation of EL-4 cells.Reverse transcript-polymerase chain reaction（RT-PCR） assay and colorimetric method was used to reveal the probable mechanism.Results ICT could inhibit the proliferation of EL-4 cells significantly in a dose-and time-dependent manner.Apoptotic cells were observed by TEM and flow cytometry analysis.RT-PCR analysis showed that the expression of bcl-2、P21 were down-regulated.Besides,Caspase-3 and Caspase-9 activity in EL-4 cells elevated remarkably.Conclusions ICT could inhibit the proliferation of EL-4 cells and induce EL-4 cells apoptosis in vitro.The probable mechanism may be down-regulate the expression of bcl-2、P21and elevate Caspase-3 and Caspase-9 activity in EL-4 cells.

关 键 词：淫羊藿素/ICT EL-4细胞 增殖 凋亡 

分 类 号：R36[医药卫生—病理学] R332[医药卫生—基础医学]